Problem: As urocortin (Ucn) is a placental peptide belonging to the corticotrophin-releasing hormone (CRH) family that modulates immune function in other biological models, this study evaluated Ucn effects on cytokines secretion from cultured human trophoblast cells.Method of study: Placentas were collected from normal term pregnancies after elective caesarean section, and primary trophoblast culture was prepared followed by the treatment of Ucn and/or CRH selective antagonists, antalarmin and astressin 2b. The anti-inflammatory cytokines IL-4 and IL-10 and the pro-inflammatory cytokine TNF-α were measured by ELISA.Results: Urocortin treatment induced a significant and dose-dependent increase of IL-4 and IL-10, whereas it did not affect TNF-α secretion. When incubated in the presence of LPS, Ucn reversed LPS-induced TNF-α release from cultured trophoblast cells, an effect that was blocked by the CRH-R2 selective antagonist, astressin 2b.Conclusion: Urocortin stimulates IL-4 and IL-10 secretion and reverses LPS-induced TNF-α release from trophoblast cells through action on CRH-R2 receptors, suggesting that this peptide may play a possible role as an anti-inflammatory agent. © 2009 John Wiley and Sons A/S. Cytokines; Inflammation; Placenta; Urocortin

Torricelli, M., Voltolini, C., Bloise, E., Biliotti, G., Giovannelli, A., DE BONIS, M., et al. (2009). Urocortin increases IL-4 and IL-10 secretion and reverses LPS-induced TNF-alpha release from human trophoblast primary cells. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 62(4), 224-231 [10.1111/j.1600-0897.2009.00729.x].

Urocortin increases IL-4 and IL-10 secretion and reverses LPS-induced TNF-alpha release from human trophoblast primary cells

TORRICELLI, M.;VOLTOLINI, C.;BLOISE, E.;BILIOTTI, G.;GIOVANNELLI, A.;DE BONIS, M.;IMPERATORE, A.;PETRAGLIA, F.
2009

Abstract

Problem: As urocortin (Ucn) is a placental peptide belonging to the corticotrophin-releasing hormone (CRH) family that modulates immune function in other biological models, this study evaluated Ucn effects on cytokines secretion from cultured human trophoblast cells.Method of study: Placentas were collected from normal term pregnancies after elective caesarean section, and primary trophoblast culture was prepared followed by the treatment of Ucn and/or CRH selective antagonists, antalarmin and astressin 2b. The anti-inflammatory cytokines IL-4 and IL-10 and the pro-inflammatory cytokine TNF-α were measured by ELISA.Results: Urocortin treatment induced a significant and dose-dependent increase of IL-4 and IL-10, whereas it did not affect TNF-α secretion. When incubated in the presence of LPS, Ucn reversed LPS-induced TNF-α release from cultured trophoblast cells, an effect that was blocked by the CRH-R2 selective antagonist, astressin 2b.Conclusion: Urocortin stimulates IL-4 and IL-10 secretion and reverses LPS-induced TNF-α release from trophoblast cells through action on CRH-R2 receptors, suggesting that this peptide may play a possible role as an anti-inflammatory agent. © 2009 John Wiley and Sons A/S. Cytokines; Inflammation; Placenta; Urocortin
Torricelli, M., Voltolini, C., Bloise, E., Biliotti, G., Giovannelli, A., DE BONIS, M., et al. (2009). Urocortin increases IL-4 and IL-10 secretion and reverses LPS-induced TNF-alpha release from human trophoblast primary cells. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 62(4), 224-231 [10.1111/j.1600-0897.2009.00729.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/6461
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