The binding of cis-diamminedichloroplatinum(II) (cis-DDP) to DNA is investigated as a function of the GC content and of the cis-DDP concentration. Analysis of the melting curves and of the UV and CD spectra shows that binding of cis-DDP to DNA is highly sequence specific, GC rich DNAs being destabilized more effectively. At low levels of platination, stabilization of GC- and destabilization of AT-base pairs can be noted. In AT rich DNA, cis-DDP binding favours the B --> A conformational transition. It is also shown that, in addition to specific interactions between cis-DDP and DNA, several non-specific processes take place.
Haroutiunian, S.G., Bruni, B., Monnanni, R., Orioli, P., Mangani, S. (1991). The Dependence of Cis-diamminedichloroplatinum(ii) Binding To Dna Upon the Gc Content - A Thermal and Spectrophotometric Investigation. INORGANICA CHIMICA ACTA, 184(1), 127-132 [10.1016/S0020-1693(00)83054-6].
The Dependence of Cis-diamminedichloroplatinum(ii) Binding To Dna Upon the Gc Content - A Thermal and Spectrophotometric Investigation
Mangani S.
1991-01-01
Abstract
The binding of cis-diamminedichloroplatinum(II) (cis-DDP) to DNA is investigated as a function of the GC content and of the cis-DDP concentration. Analysis of the melting curves and of the UV and CD spectra shows that binding of cis-DDP to DNA is highly sequence specific, GC rich DNAs being destabilized more effectively. At low levels of platination, stabilization of GC- and destabilization of AT-base pairs can be noted. In AT rich DNA, cis-DDP binding favours the B --> A conformational transition. It is also shown that, in addition to specific interactions between cis-DDP and DNA, several non-specific processes take place.
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https://hdl.handle.net/11365/34597
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