TEM-72, a class A beta-lactamase identified in isolates of Enterobacteriaceae, is a quadruple mutant of TEM-1 (Q39K, M182T, G238S and E240K) and shows extended-spectrum beta-lactamase (ESBL) properties arising from the G238S and E240K substitutions. Although many structures of TEM variants have been published, they do not include an enzyme with the simultaneous presence of both of the ESBL-conferring G238S and E240K substitutions. Furthermore, the structure shows the presence of a citrate anion bound to the TEM-72 active site, where it interacts with all of the conserved residues of class A beta-lactamases. The present structure supports the use of polycarboxylates as a scaffold for the design of broad-spectrum inhibitors of serine beta-lactamases.
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|Titolo:||Structure of the extended-spectrum beta-lactamase TEM-72 inhibited by citrate|
|Citazione:||Docquier, J.D., Benvenuti, M., V., C., Rossolini, G.M., & Mangani, S. (2011). Structure of the extended-spectrum beta-lactamase TEM-72 inhibited by citrate. ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 67(3), 303-306.|
|Appare nelle tipologie:||1.1 Articolo in rivista|