A porous multi-layered composite hydrogel (MSC), made of poly(vinyl alcohol) (PVA) and hydroxyapatite (HA), suitable as substitute for damaged cartilage, has been modified by the production of pores and its cytocompatibility and ability to prevent chondrocyte dedifferentiation in in vitro cell culture systems have been evaluated. Pores resulted homogeneously distributed on all the hydrogel surface and bulk. The material was not able to compromise cell viability, proliferation and structure of both NIH3T3 mouse fibroblasts and human chondrocytes (HC), supported HC colonization and showed a good ability to stimulate the production of hyaline extracellular matrix (ECM).
Lamponi, S., Leone, G., Consumi, M., Nelli, N., Magnani, A. (2021). Porous multi-layered composite hydrogel as cell substrate for in vitro culture of chondrocytes. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, 70(11), 764-773 [10.1080/00914037.2020.1765351].
Porous multi-layered composite hydrogel as cell substrate for in vitro culture of chondrocytes
Lamponi S.;Leone G.;Consumi M.;Nelli N.;Magnani A.
2021-01-01
Abstract
A porous multi-layered composite hydrogel (MSC), made of poly(vinyl alcohol) (PVA) and hydroxyapatite (HA), suitable as substitute for damaged cartilage, has been modified by the production of pores and its cytocompatibility and ability to prevent chondrocyte dedifferentiation in in vitro cell culture systems have been evaluated. Pores resulted homogeneously distributed on all the hydrogel surface and bulk. The material was not able to compromise cell viability, proliferation and structure of both NIH3T3 mouse fibroblasts and human chondrocytes (HC), supported HC colonization and showed a good ability to stimulate the production of hyaline extracellular matrix (ECM).File | Dimensione | Formato | |
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https://hdl.handle.net/11365/1140257