The aim of this work was the functional and proteomic analysis of a mutant, W3110 Bgl+/10, isolated from a batch culture of an Escherichia coli K-12 strain maintained at room temperature without addition of nutrients for 10 years. When the mutant was evaluated in competition experiments in co-culture with the wild-type, it exhibited the growth advantage in stationary phase (GASP) phenotype. Proteomes of the GASP mutant and its parental strain were compared by using a 2DE coupled with MS approach. Several differentially expressed proteins were detected and many of them were successful identified by mass spectrometry. Identified expression-changing proteins were grouped into three functional categories: metabolism, protein synthesis, chaperone and stress responsive proteins. Among them, the prevalence was ascribable to the “metabolism” group (72%) for the GASP mutant, and to “chaperones and stress responsive proteins” group for the parental strain (48% ).

Gagliardi, A., Lamboglia, E., Bianchi, L., Landi, C., Armini, A., Ciolfi, S., et al. (2016). Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype. PROTEOMICS, 16(6), 963-972 [10.1002/pmic.201500314].

Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype.

Gagliardi, Assunta;Bianchi, Laura;Landi, Claudia;Armini, Alessandro;Ciolfi, Silvia;Bini, Luca;Marri, Laura
2016-01-01

Abstract

The aim of this work was the functional and proteomic analysis of a mutant, W3110 Bgl+/10, isolated from a batch culture of an Escherichia coli K-12 strain maintained at room temperature without addition of nutrients for 10 years. When the mutant was evaluated in competition experiments in co-culture with the wild-type, it exhibited the growth advantage in stationary phase (GASP) phenotype. Proteomes of the GASP mutant and its parental strain were compared by using a 2DE coupled with MS approach. Several differentially expressed proteins were detected and many of them were successful identified by mass spectrometry. Identified expression-changing proteins were grouped into three functional categories: metabolism, protein synthesis, chaperone and stress responsive proteins. Among them, the prevalence was ascribable to the “metabolism” group (72%) for the GASP mutant, and to “chaperones and stress responsive proteins” group for the parental strain (48% ).
Gagliardi, A., Lamboglia, E., Bianchi, L., Landi, C., Armini, A., Ciolfi, S., et al. (2016). Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype. PROTEOMICS, 16(6), 963-972 [10.1002/pmic.201500314].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/984121