The identification of reproductive toxicants is a major scientific challenge for human health. We investigated the effects of a selected group of environmental polluting chemicals mostly provided with estrogenic activity on the human trophoblast cell lines BeWo and HTR-8/SVneo. Cells were exposed for 24 hours to various concentrations (from 0.1 pM to 1 mM) of atrazine (ATR), diethylstilbestrol (DES), para-nonylphenol (p-NP), resveratrol (RES) and 17 β-estradiol (E2) and assayed for cell viability and human beta- Chorionic Gonadotropin (β-hCG) secretion. Decrease of cell viability as respect to control, vehicle-treated, cultures was obtained for all chemicals in the concentration range of 1 μM – 1 mM in both cell types. A parallel decrease of β-hCG secretion was observed in BeWo cells, at 1 μM – 1 mM concentrations, with the only exception of ATR which caused an increase at concentrations up to 1 mM. β-hCG release was also unexpectedly inhibited by ATR, DES, p-NP and RES at non-toxic (pM-nM) concentrations These findings raise concern about the negative, potential effects of various environmental polluting chemicals on pregnancy success and fetal health.
|Titolo:||Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol|
|Citazione:||Bechi, N., Sorda, G., Spagnoletti, A., Bhattacharjee, J., Vieira Ferro, E.a., De Freitas Barbosa, B., et al. (2013). Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol. TOXICOLOGY IN VITRO, 27(3), 995-1000.|
|Appare nelle tipologie:||1.1 Articolo in rivista|