The present study was aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit heart hypoperfused with RBC suspensions under 30 mm Hg constant pressure. RBC or C-RBC caused a rapid and marked reduction of CF, CFo and HR. In RBC-treated hearts, however, reperfusion with Tyrode solution partially restored the initial myocardial parameters, while in C-RBC-treated hearts a rapid impairment of diastolic relaxation with a subsequent, steady and increasing heart contracture was observed. Histological analysis showed that in C-RBC-perfused hearts either capillaries or precapillary arterioles were occluded by C-RBC in spite of extensive washings with Tyrode solution. These findings indicate that C-RBC impair coronary circulation markedly and irreversibly.

Frosini, M., Larini, A., Ricci, L., Lucas, L., Gorelli, B., Sgaragli, G.P., et al. (2012). Effects of Autologous, Cross-Linked Erythrocytes on Isolated Hypoperfused Rabbit Heart Dynamics. PHARMACOLOGY, 90(5-6), 274-280 [10.1159/000341910].

Effects of Autologous, Cross-Linked Erythrocytes on Isolated Hypoperfused Rabbit Heart Dynamics

FROSINI, MARIA;LARINI, ALESSANDRA;RICCI, LORENZO;SGARAGLI, GIAN PIETRO;TANGANELLI, PIERO;VALOTI, MASSIMO
2012-01-01

Abstract

The present study was aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit heart hypoperfused with RBC suspensions under 30 mm Hg constant pressure. RBC or C-RBC caused a rapid and marked reduction of CF, CFo and HR. In RBC-treated hearts, however, reperfusion with Tyrode solution partially restored the initial myocardial parameters, while in C-RBC-treated hearts a rapid impairment of diastolic relaxation with a subsequent, steady and increasing heart contracture was observed. Histological analysis showed that in C-RBC-perfused hearts either capillaries or precapillary arterioles were occluded by C-RBC in spite of extensive washings with Tyrode solution. These findings indicate that C-RBC impair coronary circulation markedly and irreversibly.
Frosini, M., Larini, A., Ricci, L., Lucas, L., Gorelli, B., Sgaragli, G.P., et al. (2012). Effects of Autologous, Cross-Linked Erythrocytes on Isolated Hypoperfused Rabbit Heart Dynamics. PHARMACOLOGY, 90(5-6), 274-280 [10.1159/000341910].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/42455
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