The aim of the present investigation was to assess neuroprotection exerted by diazepam (0.1-25 microM) in rat cortical brain slices subjected to oxygen-glucose deprivation and reoxygenation. Neuronal injury and neuroprotection were assessed by measuring the release of glutamate and lactate dehydrogenase and tissue water content. Results demonstrate that diazepam exerted neuroprotective effects according to a "U-shaped", hormetic-like, concentration-response curve, with an efficacy window of 0.5-5 microM concentration. Flumazenil (20 microM) fully antagonised neuroprotection afforded by 5 microM diazepam. In conclusion, the hormetic response of diazepam should be taken into consideration when designing experiments aimed at assessing diazepam neuroprotection against ischemia/reoxygenation injury.

Ricci, L., Valoti, M., Sgaragli, G.P., Frosini, M. (2007). Neuroprotection afforded by diazepam against oxygen/glucose deprivation-induced injury in rat cortical brain slices. EUROPEAN JOURNAL OF PHARMACOLOGY, 561(1-2), 80-84 [10.1016/j.ejphar.2006.12.030].

Neuroprotection afforded by diazepam against oxygen/glucose deprivation-induced injury in rat cortical brain slices

RICCI, LORENZO;VALOTI, MASSIMO;SGARAGLI, GIAN PIETRO;FROSINI, MARIA
2007-01-01

Abstract

The aim of the present investigation was to assess neuroprotection exerted by diazepam (0.1-25 microM) in rat cortical brain slices subjected to oxygen-glucose deprivation and reoxygenation. Neuronal injury and neuroprotection were assessed by measuring the release of glutamate and lactate dehydrogenase and tissue water content. Results demonstrate that diazepam exerted neuroprotective effects according to a "U-shaped", hormetic-like, concentration-response curve, with an efficacy window of 0.5-5 microM concentration. Flumazenil (20 microM) fully antagonised neuroprotection afforded by 5 microM diazepam. In conclusion, the hormetic response of diazepam should be taken into consideration when designing experiments aimed at assessing diazepam neuroprotection against ischemia/reoxygenation injury.
Ricci, L., Valoti, M., Sgaragli, G.P., Frosini, M. (2007). Neuroprotection afforded by diazepam against oxygen/glucose deprivation-induced injury in rat cortical brain slices. EUROPEAN JOURNAL OF PHARMACOLOGY, 561(1-2), 80-84 [10.1016/j.ejphar.2006.12.030].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/412220
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo