A 100-kDa polypeptide with microtubule-interacting properties was identified in a Golgi vesicle-enriched fraction from Corylus avellana pollen. The k71s23 antibody (directed to the kinesin heavy chain from bovine brain) [Tiezzi et al., 1992: Cell Motil. Cytoskeleton 21:132- 137] localized the polypeptide on the external surface of membrane- bounded organelles. Some 100-kDa-containing vesicles copelleted with microtubules (polymerized from purified bovine brain tubulin) either in presence or absence of 5 mM AMPPNP, but they could be released by 10 mM ATP or 0.5 M KCl. The pollen microtubule-interacting protein, salt- extracted from membranes and partially purified by gel filtration, exhibited an ATPase activity (16.2 nmolPi/mg/min) which could be stimulated about 2-fold (32.5 nmolPi/mg/min) by addition of bovine brain microtubules. We suppose that the 100-kDa polypeptide is part of a molecular complex showing properties of the kinesin class

Liu, G.Q., Cai, G., DEL CASINO, C., Tiezzi, A., Cresti, M. (1994). Kinesin-related polypeptide is associated with vesicles from Corylus avellana pollen. CELL MOTILITY AND THE CYTOSKELETON, 29(2), 155-166 [10.1002/cm.970290207].

Kinesin-related polypeptide is associated with vesicles from Corylus avellana pollen

CAI, GIAMPIERO;DEL CASINO, CECILIA;CRESTI, MAURO
1994-01-01

Abstract

A 100-kDa polypeptide with microtubule-interacting properties was identified in a Golgi vesicle-enriched fraction from Corylus avellana pollen. The k71s23 antibody (directed to the kinesin heavy chain from bovine brain) [Tiezzi et al., 1992: Cell Motil. Cytoskeleton 21:132- 137] localized the polypeptide on the external surface of membrane- bounded organelles. Some 100-kDa-containing vesicles copelleted with microtubules (polymerized from purified bovine brain tubulin) either in presence or absence of 5 mM AMPPNP, but they could be released by 10 mM ATP or 0.5 M KCl. The pollen microtubule-interacting protein, salt- extracted from membranes and partially purified by gel filtration, exhibited an ATPase activity (16.2 nmolPi/mg/min) which could be stimulated about 2-fold (32.5 nmolPi/mg/min) by addition of bovine brain microtubules. We suppose that the 100-kDa polypeptide is part of a molecular complex showing properties of the kinesin class
1994
Liu, G.Q., Cai, G., DEL CASINO, C., Tiezzi, A., Cresti, M. (1994). Kinesin-related polypeptide is associated with vesicles from Corylus avellana pollen. CELL MOTILITY AND THE CYTOSKELETON, 29(2), 155-166 [10.1002/cm.970290207].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/40658