Objectives:Endocrine disrupter chemicals (EDCs) (e.g. herbicides, fungicides, insecti-cides, industrial chemicals) are environmental pollutants of agricultural or industrial or-igin which may influence human reproductive health. We recently found that para-nonylphenol (p-NP), a metabolite of alkylphenols widely used in chemicals and manufac-turer industries, induces trophoblast differentiation and apoptosis on first trimester humanplacental tissues. We hypothesized that para-nonylphenol may also influence on the cy-tokine production. The aim of the present study is to analyze the effects of p-NP on cy-tokines production by in vitro cultures of chorionic villous explants from first trimesterhuman placenta.Methods:Chorionic villous explants were cultured and treated with p-NP (10-13, 10-11,10-9 M) or only with vehicle (ethanol 0.1%) as control. Culture supernatants were col-lected after 24 hours and assayed by specific BioSurce’s Multiplex Bead Immunoassayfor the cytokines GM-CSF, IL-2, IL-6, TNF-a, IFN-g, IL-4, IL-8, IL-1b, IL-5 and IL-10. The cytokine MIF was assayed by a colorimetric sandwich assay (ELISA).Results:p-NP modulated cytokines secretion by inducing the release of GM-CSF, IL-6,IFN-g, IL-4, IL-8 and IL-1bat 10-9 M concentration while reducing release of MIF at allthree concentrations. Level of IL-2, IL-5, IL10 and TNF? was remained under the detec-tion limit both in treated and untreated control cultures.Conclusion:During the peri-implantation period, a multidirectional cytokine network isnecessary for blastocyst implantation and embryonic development. An unbalanced cyto-kine network may result in implantation failure, pregnancy loss and/or other complica-tions. Our findings on the effects of p-NP on cytokine release raised considerableconcern about the implications of maternal exposure to this chemical duringpregnancy.

Bechi, N., Romagnoli, R., Sorda, G., Corsi, I., Serchi, T., Cenicini, M., et al. (2008). Effect of para-nonylphenol on cytokine secretion in first trimester human placenta. PLACENTA, 29(8), A22-A22 [https://doi.org/10.1016/j.placenta.2008.06.002].

Effect of para-nonylphenol on cytokine secretion in first trimester human placenta

BECHI, N.;ROMAGNOLI, R.;SORDA, G.;CORSI, I.;SERCHI, T.;GALASSI, G.;RICCI, L.;IETTA, F.
2008-01-01

Abstract

Objectives:Endocrine disrupter chemicals (EDCs) (e.g. herbicides, fungicides, insecti-cides, industrial chemicals) are environmental pollutants of agricultural or industrial or-igin which may influence human reproductive health. We recently found that para-nonylphenol (p-NP), a metabolite of alkylphenols widely used in chemicals and manufac-turer industries, induces trophoblast differentiation and apoptosis on first trimester humanplacental tissues. We hypothesized that para-nonylphenol may also influence on the cy-tokine production. The aim of the present study is to analyze the effects of p-NP on cy-tokines production by in vitro cultures of chorionic villous explants from first trimesterhuman placenta.Methods:Chorionic villous explants were cultured and treated with p-NP (10-13, 10-11,10-9 M) or only with vehicle (ethanol 0.1%) as control. Culture supernatants were col-lected after 24 hours and assayed by specific BioSurce’s Multiplex Bead Immunoassayfor the cytokines GM-CSF, IL-2, IL-6, TNF-a, IFN-g, IL-4, IL-8, IL-1b, IL-5 and IL-10. The cytokine MIF was assayed by a colorimetric sandwich assay (ELISA).Results:p-NP modulated cytokines secretion by inducing the release of GM-CSF, IL-6,IFN-g, IL-4, IL-8 and IL-1bat 10-9 M concentration while reducing release of MIF at allthree concentrations. Level of IL-2, IL-5, IL10 and TNF? was remained under the detec-tion limit both in treated and untreated control cultures.Conclusion:During the peri-implantation period, a multidirectional cytokine network isnecessary for blastocyst implantation and embryonic development. An unbalanced cyto-kine network may result in implantation failure, pregnancy loss and/or other complica-tions. Our findings on the effects of p-NP on cytokine release raised considerableconcern about the implications of maternal exposure to this chemical duringpregnancy.
Bechi, N., Romagnoli, R., Sorda, G., Corsi, I., Serchi, T., Cenicini, M., et al. (2008). Effect of para-nonylphenol on cytokine secretion in first trimester human placenta. PLACENTA, 29(8), A22-A22 [https://doi.org/10.1016/j.placenta.2008.06.002].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/36793
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo