Carbohydrate microarrays are essential tools to determine functions of glycans. In this work we analysed the applicability of a four-step procedure, previously used to monolayers [G. Leone, M. Consumi, A. Tognazzi, A. Magnani, Realisation and chemical characterisation of a model system for saccharide-based biosensor, Thin Solid Films 519 (2010) 462–470], for the realisation of micropatterned substrates. The applicability was demonstrated by a combination of static time of flight secondary ion mass spectrometry and infrared reflection–adsorption spectroscopy (ToF-SIMS) which permitted not only to confirm the functionalisation of the substrates but also to detect the orientation of bound molecules. The ToF-SIMS raw data were also analysed by principal component analysis and cluster analysis to minimise the bias and confirm the validity of the results. The discriminating action of the modified supports versus cell adhesion was also demonstrated.

Leone, G., Consumi, M., Lamponi, S., Magnani, A. (2012). Combination of static time of flight secondary ion mass spectrometry andinfrared reflection–adsorption spectroscopy for the characterisation of a foursteps built-up carbohydrate array. APPLIED SURFACE SCIENCE, 258(17), 6302-6315 [10.1016/j.apsusc.2012.03.027].

Combination of static time of flight secondary ion mass spectrometry andinfrared reflection–adsorption spectroscopy for the characterisation of a foursteps built-up carbohydrate array

LEONE, GEMMA;Consumi, Marco;LAMPONI, STEFANIA;MAGNANI, AGNESE
2012-01-01

Abstract

Carbohydrate microarrays are essential tools to determine functions of glycans. In this work we analysed the applicability of a four-step procedure, previously used to monolayers [G. Leone, M. Consumi, A. Tognazzi, A. Magnani, Realisation and chemical characterisation of a model system for saccharide-based biosensor, Thin Solid Films 519 (2010) 462–470], for the realisation of micropatterned substrates. The applicability was demonstrated by a combination of static time of flight secondary ion mass spectrometry and infrared reflection–adsorption spectroscopy (ToF-SIMS) which permitted not only to confirm the functionalisation of the substrates but also to detect the orientation of bound molecules. The ToF-SIMS raw data were also analysed by principal component analysis and cluster analysis to minimise the bias and confirm the validity of the results. The discriminating action of the modified supports versus cell adhesion was also demonstrated.
Leone, G., Consumi, M., Lamponi, S., Magnani, A. (2012). Combination of static time of flight secondary ion mass spectrometry andinfrared reflection–adsorption spectroscopy for the characterisation of a foursteps built-up carbohydrate array. APPLIED SURFACE SCIENCE, 258(17), 6302-6315 [10.1016/j.apsusc.2012.03.027].
File in questo prodotto:
File Dimensione Formato  
Applied Surface Science_2012.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 1.97 MB
Formato Adobe PDF
1.97 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/32920
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo