Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.

Bernini, A., Spiga, O., Clutti, A., Venditti, V., Prischi, F., Governatori, M., et al. (2006). NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1764(5), 856-862 [10.1016/j.bbapap.2006.02.013].

NMR studies of BPTI aggregation by using paramagnetic relaxation reagents

Bernini, Andrea;Spiga, Ottavia;Bracci, Luisa;Laschi, Franco;Niccolai, Neri
2006-01-01

Abstract

Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.
2006
Bernini, A., Spiga, O., Clutti, A., Venditti, V., Prischi, F., Governatori, M., et al. (2006). NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1764(5), 856-862 [10.1016/j.bbapap.2006.02.013].
File in questo prodotto:
File Dimensione Formato  
bba_2006.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 398.63 kB
Formato Adobe PDF
398.63 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/3154
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo