There is evidence that alpha-smooth muscle actin (alpha-SMA) is a protein that plays a pivotal role in the production of contractile forces and it is induced by transforming growth factor-beta1 (TGF-beta1). We have analysed the expression of alpha-SMA, TGF-beta1, its receptor RI and the activator phospho-Smad2 in (a) fetal growth restriction pre-eclamptic placentae characterised by early onset and absence of end diastolic velocities in the umbilical arteries (FGR-AED) and (b) control placentae accurately matched for gestational age. The study was performed by immunohistochemical, quantitative Western blotting, ELISA, RT-PCR and in vitro analyses. We found that TGF-beta1 stimulates alpha-SMA production in chorionic villi cultured in vitro. In addition, we observed that in vivo TGF-beta1 concentration is significantly higher in FGR-AED placental samples than in control placentae and that this growth factor could have a paracrine action on villous stroma myofibroblasts expressing TGF-beta1 receptors and phospho-Smad2. Indeed, we report that alpha-SMA undergoes a redistribution in FGR-AED placental villous tree, i.e. we show that alpha-SMA is enhanced in medium and small stem villi and significantly decreased in the peripheral villi. Our data allow us to consider TGF-beta1 and alpha-SMA as key molecules related to FGR-AED placental villous tree phenotypic changes responsible for increased impedance to blood flow observable in this pathology.
Todros, T., Marzioni, D., Lorenzi, T., Piccoli, E., Capparuccia, L., Perugini, V., et al. (2007). Evidence for a Role of TGF-beta1 in the Expression and Regulation of alpha-SMA in Fetal Growth Restricted Placentae. PLACENTA, 28(11-12), 1123-1132.
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|Titolo:||Evidence for a Role of TGF-beta1 in the Expression and Regulation of alpha-SMA in Fetal Growth Restricted Placentae|
|Citazione:||Todros, T., Marzioni, D., Lorenzi, T., Piccoli, E., Capparuccia, L., Perugini, V., et al. (2007). Evidence for a Role of TGF-beta1 in the Expression and Regulation of alpha-SMA in Fetal Growth Restricted Placentae. PLACENTA, 28(11-12), 1123-1132.|
|Appare nelle tipologie:||1.1 Articolo in rivista|