Fibrinogen-Catecholamine Interaction as Observed by NMR and Fourier Transform Infrared Spectroscopy

In this work, the interactions between the main catecholaminessepinephrine and norepinephrinesand fibrinogen were investigated by NMR and Fourier transform infrared spectroscopies. The two hormones were found to interact with fibrinogen and to affect the protein secondary structure to a different extent. In particular, the protein selectively binds epinephrine at both the basal and stress concentrations, while it shows a weak nonspecific interaction with norepinephrine. The interaction with the stress level of epinephrine leads to drastic protein conformational changes, whereas norepinephrine does not affect fibrinogen secondary structure, even at stress concentration.