This paper reports the results of a study performed to develop a rapid and straightforward chromatographic method for the identification and quantification of trans-resveratrol in commercial trans-resveratrol-containing dietary supplements. The method employs an Alltech Platinum EPS 100 A C-18 column operated under isocratic elution mode with a 70:30 (v/v) water-acetonitrile mixture as the mobile phase at flow rate of 1.0 mL min-1. Peak detection is performed at 306 nm with limit of detection (LOD) of 0.27 μg mL-1 and limit of quantification (LOQ) of 0.95 μg mL-1 and linear calibration graph within the concentration range of 2.63 to 10.53 μg mL-1. Other data related to the successful method validation include representative linear regression equation and correlation coefficient of the calibration graph for quantification of trans-resveratrol, as well as the results of replicate analysis of real samples and of a recovery study, carried out to evaluate precision and accuracy, respectively. The method is successfully applied to study the stability of trans-resveratrol in methanol solution under various storage conditions and to analyse commercial preparations of trans-resveratrol-containing dietary supplements.

Brizzi, V., Corradini, D., Brizzi, A. (2008). Identification and Quantification of Trans-Resveratrol in Dietary Supplements by a Rapid and Straightforward RP-HPLC Method. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 31(14), 2089-2100 [10.1080/10826070802225353].

Identification and Quantification of Trans-Resveratrol in Dietary Supplements by a Rapid and Straightforward RP-HPLC Method

Brizzi, Vittorio;Brizzi, Antonella
2008-01-01

Abstract

This paper reports the results of a study performed to develop a rapid and straightforward chromatographic method for the identification and quantification of trans-resveratrol in commercial trans-resveratrol-containing dietary supplements. The method employs an Alltech Platinum EPS 100 A C-18 column operated under isocratic elution mode with a 70:30 (v/v) water-acetonitrile mixture as the mobile phase at flow rate of 1.0 mL min-1. Peak detection is performed at 306 nm with limit of detection (LOD) of 0.27 μg mL-1 and limit of quantification (LOQ) of 0.95 μg mL-1 and linear calibration graph within the concentration range of 2.63 to 10.53 μg mL-1. Other data related to the successful method validation include representative linear regression equation and correlation coefficient of the calibration graph for quantification of trans-resveratrol, as well as the results of replicate analysis of real samples and of a recovery study, carried out to evaluate precision and accuracy, respectively. The method is successfully applied to study the stability of trans-resveratrol in methanol solution under various storage conditions and to analyse commercial preparations of trans-resveratrol-containing dietary supplements.
2008
Brizzi, V., Corradini, D., Brizzi, A. (2008). Identification and Quantification of Trans-Resveratrol in Dietary Supplements by a Rapid and Straightforward RP-HPLC Method. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 31(14), 2089-2100 [10.1080/10826070802225353].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/24110
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