We describe the group selective separation and quantification of unmodified and modified purines in human urine by high-performance reverse phase liquid chromatography. The pattern of oxypurines and methylated purines: hypoxanthine (Hx), xanthine (X), 1-methyl hypoxanthine (1-MHx), 1-methyl guanine (1-MG), 3-methyl guanine (3-MG), 7-methyl guanine (7-MG), 1-methyl xanthine (1-MX), 3-methyl xanthine (3-MX), 7-methyl xanthine (7-MX), 1,7-dimethyl guanine (1,7-dMG), 1,3-dimethyl xanthine (1,3-dMX), 1,7-dimethyl xanthine (3,7-dMX) and 1,3,7-trimethyl xanthine (1,3,7-tMX) were determined in a single run in urine of a healthy subject and a gout patient before and after treatment with allopurinol. This method may be useful to investigate the urinary pattern of methylated bases in diseases involving purine metabolism.
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|Titolo:||Determination of Urinary Methylated Purine Pattern by High Performance Liquid Chromatography|
|Citazione:||Di Pietro, M.C., Vannoni, D., Leoncini, R., Liso, G., Guerranti, R., & Marinello, E. (2001). Determination of Urinary Methylated Purine Pattern by High Performance Liquid Chromatography. JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL SCIENCES AND APPLICATIONS, 751(1), 87-92.|
|Appare nelle tipologie:||1.1 Articolo in rivista|