In this paper, we demonstrate the existence and localization of fucosyl-containing O-glycoforms of nucleolin in cultured bovine endothelial cells (CVEC) and malignant cultured human A431 cells. The tool for this discovery was an antibody raised against gp273, a glycoprotein ligand for the sperm–egg interaction in the mollusc bivalve Unio elongatulus. The function and immunological properties of gp273 mainly depend on clustered Lewis-like, fucose-containing O-glycans. Here an anti-gp273 antibody was used to evaluate whether glycoepitopes similar to those of gp273 are part of potential ligands of selectins in endothelial cells.We found that anti-gp273 strongly and exclusively interacted with a 110 kDa protein in CVEC and A431 tumor cells. After partial purification, mass spectrometry identified the protein as nucleolin. This was confirmed by comparing anti-gp273 and anti-nucleolin antibody immunoblotting after nucleolin depletion.We confirmed that anti-gp273 binding to nuclear and extranuclear nucleolin was against a fucose-containing O-glycoepitope by immunoblot analysis of the protein after chemically removing O-glycans and by lectin-blot analysis of control and nucleolin-depleted samples. Using anti-gp273 IgG, we detected nucleolin on the plasma membrane and cytoplasm. O-glycosylation may regulate the plethora of functions in which nucleolin is involved.

Aldi, S., DELLA GIOVAMPAOLA, C., Focarelli, R., Armini, A., Ziche, M., Finetti, F., et al. (2009). A fucose-containing O-glycoepitope on bovine and human nucleolin. GLYCOBIOLOGY, 19(4), 337-343 [10.1093/glycob/cwn126].

A fucose-containing O-glycoepitope on bovine and human nucleolin

ALDI, SILVIA;DELLA GIOVAMPAOLA, CINZIA;FOCARELLI, RICCARDO;ARMINI, ALESSANDRO;ZICHE, MARINA;FINETTI, FEDERICA;ROSATI, FLORIANA
2009-01-01

Abstract

In this paper, we demonstrate the existence and localization of fucosyl-containing O-glycoforms of nucleolin in cultured bovine endothelial cells (CVEC) and malignant cultured human A431 cells. The tool for this discovery was an antibody raised against gp273, a glycoprotein ligand for the sperm–egg interaction in the mollusc bivalve Unio elongatulus. The function and immunological properties of gp273 mainly depend on clustered Lewis-like, fucose-containing O-glycans. Here an anti-gp273 antibody was used to evaluate whether glycoepitopes similar to those of gp273 are part of potential ligands of selectins in endothelial cells.We found that anti-gp273 strongly and exclusively interacted with a 110 kDa protein in CVEC and A431 tumor cells. After partial purification, mass spectrometry identified the protein as nucleolin. This was confirmed by comparing anti-gp273 and anti-nucleolin antibody immunoblotting after nucleolin depletion.We confirmed that anti-gp273 binding to nuclear and extranuclear nucleolin was against a fucose-containing O-glycoepitope by immunoblot analysis of the protein after chemically removing O-glycans and by lectin-blot analysis of control and nucleolin-depleted samples. Using anti-gp273 IgG, we detected nucleolin on the plasma membrane and cytoplasm. O-glycosylation may regulate the plethora of functions in which nucleolin is involved.
Aldi, S., DELLA GIOVAMPAOLA, C., Focarelli, R., Armini, A., Ziche, M., Finetti, F., et al. (2009). A fucose-containing O-glycoepitope on bovine and human nucleolin. GLYCOBIOLOGY, 19(4), 337-343 [10.1093/glycob/cwn126].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21302
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