Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate gene expression of adenosine kinase, a key enzyme in adenosine metabolism, in human intestinal biopsy specimens of 10 colorectal cancer patients. Quantitative mRNA expression levels were normalized against the reference gene beta-actin. The results showed that adenosine kinase gene expression was significantly higher in cancer than in normal-appearing tissue, in line with our previous measurements of adenosine kinase enzyme activities in colorectal tumor samples.

Giglioni, S., Leoncini, R., Aceto, E., Chessa, A., Civitelli, S., Bernini, A., et al. (2008). Adenosine kinase gene expression in human colorectal cancer. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 27(6-7), 750-754 [10.1080/15257770802145629].

Adenosine kinase gene expression in human colorectal cancer.

GIGLIONI, STEFANIA;LEONCINI, ROBERTO;ACETO, EMILIA;CHESSA, ANTONELLA;CIVITELLI, SERENELLA;TANZINI, GABRIELLO;CARRARO, FABIO;PUCCI, ANNALISA;VANNONI, DANIELA
2008-01-01

Abstract

Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate gene expression of adenosine kinase, a key enzyme in adenosine metabolism, in human intestinal biopsy specimens of 10 colorectal cancer patients. Quantitative mRNA expression levels were normalized against the reference gene beta-actin. The results showed that adenosine kinase gene expression was significantly higher in cancer than in normal-appearing tissue, in line with our previous measurements of adenosine kinase enzyme activities in colorectal tumor samples.
Giglioni, S., Leoncini, R., Aceto, E., Chessa, A., Civitelli, S., Bernini, A., et al. (2008). Adenosine kinase gene expression in human colorectal cancer. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 27(6-7), 750-754 [10.1080/15257770802145629].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/19088
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