X-ray structures of homopolymeric human L-ferritin and horse spleen ferritin were solved by freezing protein crystals at different time intervals after exposure to a ferric salt and revealed the growth of an octa-nuclear iron cluster on the inner surface of the protein cage with a key role played by some glutamate residues. An atomic resolution view of how the cluster formation develops starting from a (μ3-oxo)tris[(μ2-glutamato-κO:κO’)](glutamato-κO)(diaquo)triiron(III) seed is provided. The results support the idea that iron biomineralization in ferritin is a process initiating at the level of the protein surface, capable of contributing coordination bonds and electrostatic guidance.
Ciambellotti, S., Pozzi, C., Mangani, S., Turano, P. (2020). Iron Biomineral Growth from the Initial Nucleation Seed in L-Ferritin. CHEMISTRY-A EUROPEAN JOURNAL, 26(26), 5770-5773 [10.1002/chem.202000064].
Iron Biomineral Growth from the Initial Nucleation Seed in L-Ferritin
Pozzi C.;Mangani S.
;
2020-01-01
Abstract
X-ray structures of homopolymeric human L-ferritin and horse spleen ferritin were solved by freezing protein crystals at different time intervals after exposure to a ferric salt and revealed the growth of an octa-nuclear iron cluster on the inner surface of the protein cage with a key role played by some glutamate residues. An atomic resolution view of how the cluster formation develops starting from a (μ3-oxo)tris[(μ2-glutamato-κO:κO’)](glutamato-κO)(diaquo)triiron(III) seed is provided. The results support the idea that iron biomineralization in ferritin is a process initiating at the level of the protein surface, capable of contributing coordination bonds and electrostatic guidance.File | Dimensione | Formato | |
---|---|---|---|
2020 Chemistry L-ferritins cluster accepted manuscript.pdf
accesso aperto
Descrizione: Articolo
Tipologia:
Post-print
Licenza:
PUBBLICO - Pubblico con Copyright
Dimensione
1.19 MB
Formato
Adobe PDF
|
1.19 MB | Adobe PDF | Visualizza/Apri |
Chemistry A European J - 2020 - Ciambellotti - Iron Biomineral Growth from the Initial Nucleation Seed in L‐Ferritin.pdf
non disponibili
Descrizione: Articolo
Tipologia:
PDF editoriale
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
1.03 MB
Formato
Adobe PDF
|
1.03 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/1107315