Objective. To assay anti-ganglioside antibodies (aGM1) in sera of a large cohort of European patients with systemic lupus erythematosus (SLE) to define the prevalence of these autoantibodies in SLE; to evaluate the association of aGM1with clinical manifestations and other autoantibodies found in SLE; and to search for aGM1association with HLA class II alleles. Methods. Four hundred forty-eight patients with SLE were consecutively enrolled in 8 centers from 6 European countries. All sera were tested for antinuclear antibodies by immunofluorescence on HEp-2 cells as substrate, anti-dsDNA, aGM1aCL, aβ2-glycoprotein I (aβ2-GPI) antibodies by ELISA, and antineutrophil cytoplasmic antibodies (ANCA) by immunofluorescence and by ELISA. Genomic typing for HLA class II loci was performed by polymerase chain reaction-sequence specific oligonucleotide probe method. Clinical assessment was done at the time of enrolment. Results. We found 41.9% of patients with clinical signs of neuropsychiatric involvement; 15.5% of patients were positive for aGM:1, 8% of the IgG isotype and 8.6% of the IgM isotype; aGM1- IgG were associated with neuropsychiatric manifestations (NPM) (RR = 3.7), with migraine (RR = 2.4), with OBS (RR = 7.3), and with peripheral neuropathy (RR = 8.5). aGM1-IgM were associated with NPM (RR = 4) and with depression (RR = 3.4). Furthermore, the genetic study showed that aGM1-IgG were associated with HLA-DQB1*0404 (RR = 7.2) while aGM1-IgM were associated with HLA-DQB1*0605 (RR = 33.3). No associations were found between aGM1and anti-dsDNA, aCL, aβ2GPI, or ANCA. Conclusion. Our results show aGM1can be found in patients with SLE. aGm1may play a pathogenetic role for some NPM in this condition.

Galeazzi, M., Annunziata, P., Sebastlani, G.D., Bellisai, F., Campanella, V., Ferrara, G.B., et al. (2000). Anti-ganglioside antibodies in a large cohort of European patients with systemic lupus erythematosus: Clinical, serological, and HLA class II gene associations. THE JOURNAL OF RHEUMATOLOGY, 27(1), 135-141.

Anti-ganglioside antibodies in a large cohort of European patients with systemic lupus erythematosus: Clinical, serological, and HLA class II gene associations

Galeazzi, Mauro;Annunziata, Pasquale;Bellisai, Francesca;Bracci, Luisa;Morozzi, Gabriella;Marcolongo, Roberto
2000-01-01

Abstract

Objective. To assay anti-ganglioside antibodies (aGM1) in sera of a large cohort of European patients with systemic lupus erythematosus (SLE) to define the prevalence of these autoantibodies in SLE; to evaluate the association of aGM1with clinical manifestations and other autoantibodies found in SLE; and to search for aGM1association with HLA class II alleles. Methods. Four hundred forty-eight patients with SLE were consecutively enrolled in 8 centers from 6 European countries. All sera were tested for antinuclear antibodies by immunofluorescence on HEp-2 cells as substrate, anti-dsDNA, aGM1aCL, aβ2-glycoprotein I (aβ2-GPI) antibodies by ELISA, and antineutrophil cytoplasmic antibodies (ANCA) by immunofluorescence and by ELISA. Genomic typing for HLA class II loci was performed by polymerase chain reaction-sequence specific oligonucleotide probe method. Clinical assessment was done at the time of enrolment. Results. We found 41.9% of patients with clinical signs of neuropsychiatric involvement; 15.5% of patients were positive for aGM:1, 8% of the IgG isotype and 8.6% of the IgM isotype; aGM1- IgG were associated with neuropsychiatric manifestations (NPM) (RR = 3.7), with migraine (RR = 2.4), with OBS (RR = 7.3), and with peripheral neuropathy (RR = 8.5). aGM1-IgM were associated with NPM (RR = 4) and with depression (RR = 3.4). Furthermore, the genetic study showed that aGM1-IgG were associated with HLA-DQB1*0404 (RR = 7.2) while aGM1-IgM were associated with HLA-DQB1*0605 (RR = 33.3). No associations were found between aGM1and anti-dsDNA, aCL, aβ2GPI, or ANCA. Conclusion. Our results show aGM1can be found in patients with SLE. aGm1may play a pathogenetic role for some NPM in this condition.
Galeazzi, M., Annunziata, P., Sebastlani, G.D., Bellisai, F., Campanella, V., Ferrara, G.B., et al. (2000). Anti-ganglioside antibodies in a large cohort of European patients with systemic lupus erythematosus: Clinical, serological, and HLA class II gene associations. THE JOURNAL OF RHEUMATOLOGY, 27(1), 135-141.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1058117