Metabolism of 7-ethoxycoumarin by rat precision-cut liver slices

Precision cut liver slices are used in the study of drug metabolism and toxicity, The aim of the study is to investigate the metabolism of 7-ethoxycoumarin in precision cut liver slices. Liver slices were prepared from liver cores by Krumdieck tissue slicer. After 30 min preincubation period, the liver slices were incubated in RPMI1640 medium, t = 37°C, for 2h, 4h, 6h, 24h. 7-ethoxycoumarin 50 μM was added to the incubation medium. The viability of the preparation was evaluated by LDH release in both preincubation and incubation medium. The amount of free 7-hydroxycoumarin and its glucuronide and sulfate conjugates was measured spectrofluorometrically. The results of the study showed that the precision cut liver slices are an appropriate in vitro model for complex evaluation of phase I and phase II metabolism of 7-ethoxycoumarin.