BACKGROUND: Helicobacter pylori infection may increase the risk of both histotypes of gastric carcinoma (GC): intestinal (IGC) and diffuse (DGC). Polymorphism of the main H. pylori virulence factors, cagA and vacA, could have a different impact on the histological variety of GC. METHODS: Two hundred and twenty-six H. pylori colonies were examined from 28 GC patients: 15 with IGC and 13 with DGC. DNA was extracted from bacteria and a PCR was performed using primers specific for cagA, the 3' cagA variable region and s and m regions of vacA. RESULTS: There were 214 cagA+ strains and 55.61% of them were isolated from IGC cases; there were 12 cagA- colonies and they were all isolated from DGC (P<0.001). Most patients were infected by strains with more than one cagA structural type. No strains with a particular cagA type were found to be significantly more frequent in either histological variety of GC. A percentage of 43.90 of strains with vacA subtype s1/m1 were isolated from IGC, whereas 80.95% of vacA subtype s1/m2 strains were isolated from DGC cases (P<0.001). CONCLUSION: Polymorphism of the main virulence genes of H. pylori may play different roles in the pathogenesis of IGC and DGC.

FIGURA, N., VALASSINA, M., MORETTI, E., VINDIGNI, C., COLLODEL, G., IACOPONI, F., et al. (2015). Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 27(9), 1017-1021 [10.1097/MEG.0000000000000414].

Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism

FIGURA, NATALE;VALASSINA, MARCELLO;MORETTI, ELENA;VINDIGNI, CARLA;COLLODEL, GIULIA;IACOPONI, FRANCESCA;GIORDANO, NICOLA GIUSEPPE;MARRELLI, DANIELE
2015

Abstract

BACKGROUND: Helicobacter pylori infection may increase the risk of both histotypes of gastric carcinoma (GC): intestinal (IGC) and diffuse (DGC). Polymorphism of the main H. pylori virulence factors, cagA and vacA, could have a different impact on the histological variety of GC. METHODS: Two hundred and twenty-six H. pylori colonies were examined from 28 GC patients: 15 with IGC and 13 with DGC. DNA was extracted from bacteria and a PCR was performed using primers specific for cagA, the 3' cagA variable region and s and m regions of vacA. RESULTS: There were 214 cagA+ strains and 55.61% of them were isolated from IGC cases; there were 12 cagA- colonies and they were all isolated from DGC (P<0.001). Most patients were infected by strains with more than one cagA structural type. No strains with a particular cagA type were found to be significantly more frequent in either histological variety of GC. A percentage of 43.90 of strains with vacA subtype s1/m1 were isolated from IGC, whereas 80.95% of vacA subtype s1/m2 strains were isolated from DGC cases (P<0.001). CONCLUSION: Polymorphism of the main virulence genes of H. pylori may play different roles in the pathogenesis of IGC and DGC.
FIGURA, N., VALASSINA, M., MORETTI, E., VINDIGNI, C., COLLODEL, G., IACOPONI, F., et al. (2015). Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 27(9), 1017-1021 [10.1097/MEG.0000000000000414].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/983054
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