BACKGROUND: Helicobacter pylori infection may increase the risk of both histotypes of gastric carcinoma (GC): intestinal (IGC) and diffuse (DGC). Polymorphism of the main H. pylori virulence factors, cagA and vacA, could have a different impact on the histological variety of GC. METHODS: Two hundred and twenty-six H. pylori colonies were examined from 28 GC patients: 15 with IGC and 13 with DGC. DNA was extracted from bacteria and a PCR was performed using primers specific for cagA, the 3' cagA variable region and s and m regions of vacA. RESULTS: There were 214 cagA+ strains and 55.61% of them were isolated from IGC cases; there were 12 cagA- colonies and they were all isolated from DGC (P<0.001). Most patients were infected by strains with more than one cagA structural type. No strains with a particular cagA type were found to be significantly more frequent in either histological variety of GC. A percentage of 43.90 of strains with vacA subtype s1/m1 were isolated from IGC, whereas 80.95% of vacA subtype s1/m2 strains were isolated from DGC cases (P<0.001). CONCLUSION: Polymorphism of the main virulence genes of H. pylori may play different roles in the pathogenesis of IGC and DGC.
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|Titolo:||Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism|
|Citazione:||Figura, N., Valassina, M., Moretti, E., Vindigni, C., Collodel, G., Iacoponi, F., et al. (2015). Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 27(9), 1017-1021.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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