Toscana Virus (TOSV) is a Phlebovirus responsible for central nervous system (CNS) injury in humans. The TOSV non-structural protein (NSs), which interacting with RIG-I leads to its degradation, was analysed in the C terminus fragment in order to identify its functional domains. To this aim, two C-terminal truncated NSs proteins, Δ1C-NSs (aa 1-284) and Δ2C-NSs (aa 1-287) were tested. Only Δ1C-NSs did not present any inhibitory effect on RIG-I and it showed a greater stability than the whole NSs protein. Moreover, the deletion of the TLQ aa sequence interposed between the two ΔC constructs caused a greater accumulation of the protein with a weak inhibitory effect on RIG-I, indicating some involvement of these amino acids in the NSs activity. Nevertheless, all the truncated proteins were still able to interact with RIG-I, suggesting that the domains responsible for RIG-I signaling and RIG-I interaction are mapped on different regions of the protein.
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|Titolo:||Truncation of the C-terminal region of Toscana Virus NSs protein is critical for interferon-β antagonism and protein stability|
|Citazione:||GORI SAVELLINI, G., GANDOLFO, C., & CUSI, M.G. (2015). Truncation of the C-terminal region of Toscana Virus NSs protein is critical for interferon-β antagonism and protein stability. VIROLOGY, 486, 255-262.|
|Appare nelle tipologie:||1.1 Articolo in rivista|