Bafilomycin A1, a specific inhibitor of the vacuolar‐type H+‐ATPase, responsible for acidification of intra‐cellular compartments, prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. The vacuolating activity of Helicobacter pylori is not inhibited by a series of specific inhibitors of vacuolar H+‐ATPases. These findings indicate that a transmembrane pH gradient is needed for the formation and growth of vacuoles caused by the bacterium and that this pH gradient is due to the activity of a vacuolar ATPase proton pump of HeLa cells. Copyright © 1993, Wiley Blackwell. All rights reserved.

Papini, E., Bugnoli, M., De Bernard, M., Figura, N., Rappuoli, R., Montecucco, C. (1993). Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization on HeLa cells. MOLECULAR MICROBIOLOGY, 7(2), 323-327 [10.1111/j.1365-2958.1993.tb01123.x].

Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization on HeLa cells

Figura, N.;
1993

Abstract

Bafilomycin A1, a specific inhibitor of the vacuolar‐type H+‐ATPase, responsible for acidification of intra‐cellular compartments, prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. The vacuolating activity of Helicobacter pylori is not inhibited by a series of specific inhibitors of vacuolar H+‐ATPases. These findings indicate that a transmembrane pH gradient is needed for the formation and growth of vacuoles caused by the bacterium and that this pH gradient is due to the activity of a vacuolar ATPase proton pump of HeLa cells. Copyright © 1993, Wiley Blackwell. All rights reserved.
Papini, E., Bugnoli, M., De Bernard, M., Figura, N., Rappuoli, R., Montecucco, C. (1993). Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization on HeLa cells. MOLECULAR MICROBIOLOGY, 7(2), 323-327 [10.1111/j.1365-2958.1993.tb01123.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/9601
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