A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative-farnesoic acid O-methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative-FAMeTexpression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1- and 2-day-old females. Treated specimens were left to mate with each other or with untreated partners to evaluate the extent of each sex influencing egg production. Gene silencing was investigated by Real-Time PCR. Results unambiguously showed that FAMeT has ameasurable role on the fertility of both medfly sexes.

Vannini, L., Ciolfi, S., Dallai, R., Frati, F., Hoffmann, K., & MEYERING VOS, M. (2010). Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 75, 92-106.

Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction

VANNINI, LAURA;CIOLFI, SILVIA;DALLAI, ROMANO;FRATI, FRANCESCO;
2010

Abstract

A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative-farnesoic acid O-methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative-FAMeTexpression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1- and 2-day-old females. Treated specimens were left to mate with each other or with untreated partners to evaluate the extent of each sex influencing egg production. Gene silencing was investigated by Real-Time PCR. Results unambiguously showed that FAMeT has ameasurable role on the fertility of both medfly sexes.
Vannini, L., Ciolfi, S., Dallai, R., Frati, F., Hoffmann, K., & MEYERING VOS, M. (2010). Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 75, 92-106.
File in questo prodotto:
File Dimensione Formato  
Vannini et al., 2010b, AIBP.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 406.32 kB
Formato Adobe PDF
406.32 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/8336
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo