A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative-farnesoic acid O-methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative-FAMeTexpression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1- and 2-day-old females. Treated specimens were left to mate with each other or with untreated partners to evaluate the extent of each sex influencing egg production. Gene silencing was investigated by Real-Time PCR. Results unambiguously showed that FAMeT has ameasurable role on the fertility of both medfly sexes.
Vannini, L., Ciolfi, S., Dallai, R., Frati, F., Hoffmann, K., MEYERING VOS, M. (2010). Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 75, 92-106.
Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction
VANNINI, LAURA;CIOLFI, SILVIA;DALLAI, ROMANO;FRATI, FRANCESCO;
2010-01-01
Abstract
A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative-farnesoic acid O-methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative-FAMeTexpression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1- and 2-day-old females. Treated specimens were left to mate with each other or with untreated partners to evaluate the extent of each sex influencing egg production. Gene silencing was investigated by Real-Time PCR. Results unambiguously showed that FAMeT has ameasurable role on the fertility of both medfly sexes.
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https://hdl.handle.net/11365/8336
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