A sediment sample from Venice Lagoon was found to be contaminated with 475 mgKg1 polycyclic aromatic hydrocarbons (PAHs). Naphthalene was the principal pollutant at 26% of total PAHs. Two strains of Pseudomonas SN1 and SB1 were isolated from sediment amended with 2% naphthalene. 16S rRNA gene sequence analysis indicated that the two strains have about 99% nucleotide identity with strains of the genus Pseudomonas, and are very close to Pseudomonas stutzeri. Their metabolic profiles showed significant nutritional differences, the most significant of which was that SN1 grows in marine mineral medium spiked with naphthalene and SB1 grows with biphenyl as sole carbon and energy sources. Pseudomonas sp. SN1 had a doubling time of 3.1 h with 2% naphthalene and SB1 had a doubling time of 19.5 h with 2% biphenyl. Strain SN1 oxidised naphthalene at 564732 mgO2 l1 d1 and SB1 oxidised biphenyl at 426725 mgO2 l1 d1 in respirometry reaction vessels under controlled conditions. Screening of the two strains for dioxygenase genes involved in the first step of the two hydrocarbon degradation pathways, by polymerase chain reaction, showed naphthalene dioxygenase in SN1 and biphenyl dioxygenase in SB1. The strains each have a different catechol 2,3-dioxygenase responsible for cleavage of the aromatic ring.

Marcon, R., Bestetti, G., Frati, F., Pepi, M., & Baldi, F. (2007). Naphthalene and byphenyl oxidation by two Pseudomonas strains isolated from Venice lagoon sediment. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 59, 25-31.

Naphthalene and byphenyl oxidation by two Pseudomonas strains isolated from Venice lagoon sediment.

FRATI, FRANCESCO;PEPI, MILVA;
2007

Abstract

A sediment sample from Venice Lagoon was found to be contaminated with 475 mgKg1 polycyclic aromatic hydrocarbons (PAHs). Naphthalene was the principal pollutant at 26% of total PAHs. Two strains of Pseudomonas SN1 and SB1 were isolated from sediment amended with 2% naphthalene. 16S rRNA gene sequence analysis indicated that the two strains have about 99% nucleotide identity with strains of the genus Pseudomonas, and are very close to Pseudomonas stutzeri. Their metabolic profiles showed significant nutritional differences, the most significant of which was that SN1 grows in marine mineral medium spiked with naphthalene and SB1 grows with biphenyl as sole carbon and energy sources. Pseudomonas sp. SN1 had a doubling time of 3.1 h with 2% naphthalene and SB1 had a doubling time of 19.5 h with 2% biphenyl. Strain SN1 oxidised naphthalene at 564732 mgO2 l1 d1 and SB1 oxidised biphenyl at 426725 mgO2 l1 d1 in respirometry reaction vessels under controlled conditions. Screening of the two strains for dioxygenase genes involved in the first step of the two hydrocarbon degradation pathways, by polymerase chain reaction, showed naphthalene dioxygenase in SN1 and biphenyl dioxygenase in SB1. The strains each have a different catechol 2,3-dioxygenase responsible for cleavage of the aromatic ring.
Marcon, R., Bestetti, G., Frati, F., Pepi, M., & Baldi, F. (2007). Naphthalene and byphenyl oxidation by two Pseudomonas strains isolated from Venice lagoon sediment. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 59, 25-31.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/8226
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