Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were >100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 ± 60 kilobase pairs.

Tudor, J., Marri, L., Piggot, P.J., & DANEO MOORE, L. (1990). Size of the Streptococcus Mutans GS-5 chromosome as determined by pulsed field gel electrophoresis. INFECTION AND IMMUNITY, 58(3), 838-840.

Size of the Streptococcus Mutans GS-5 chromosome as determined by pulsed field gel electrophoresis.

MARRI, LAURA;
1990

Abstract

Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were >100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 ± 60 kilobase pairs.
Tudor, J., Marri, L., Piggot, P.J., & DANEO MOORE, L. (1990). Size of the Streptococcus Mutans GS-5 chromosome as determined by pulsed field gel electrophoresis. INFECTION AND IMMUNITY, 58(3), 838-840.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/8225
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