Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were >100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 ± 60 kilobase pairs.
Tudor, J., Marri, L., Piggot, P.J., DANEO MOORE, L. (1990). Size of the Streptococcus Mutans GS-5 chromosome as determined by pulsed field gel electrophoresis. INFECTION AND IMMUNITY, 58(3), 838-840.
Size of the Streptococcus Mutans GS-5 chromosome as determined by pulsed field gel electrophoresis.
MARRI, LAURA;
1990-01-01
Abstract
Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were >100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 ± 60 kilobase pairs.
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https://hdl.handle.net/11365/8225
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