A method for the extraction in the cold of hepatic binding protein using a novel combination of detergents, namely 1% Na urso deoxycholate and 5% Tween 80 in the extraction buffer, which allows a significantly higher recovery of lectin than 1% Triton X-100 is described. It is noteworthy that this lectin preparation can be maintained in solution for over a month and can be used in vitro and in vivo.
Pessina, G.P., Naldini, A., Bocci, V. (1986). Bulk preparation of hepatic binding protein. ITALIAN JOURNAL OF BIOCHEMISTRY, 35(1), 16-21.
Bulk preparation of hepatic binding protein
PESSINA, G. P.;NALDINI, A.;BOCCI, V.
1986-01-01
Abstract
A method for the extraction in the cold of hepatic binding protein using a novel combination of detergents, namely 1% Na urso deoxycholate and 5% Tween 80 in the extraction buffer, which allows a significantly higher recovery of lectin than 1% Triton X-100 is described. It is noteworthy that this lectin preparation can be maintained in solution for over a month and can be used in vitro and in vivo.
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https://hdl.handle.net/11365/8163
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