A method for the extraction in the cold of hepatic binding protein using a novel combination of detergents, namely 1% Na urso deoxycholate and 5% Tween 80 in the extraction buffer, which allows a significantly higher recovery of lectin than 1% Triton X-100 is described. It is noteworthy that this lectin preparation can be maintained in solution for over a month and can be used in vitro and in vivo.

Pessina, G.P., Naldini, A., & Bocci, V. (1986). Bulk preparation of hepatic binding protein. ITALIAN JOURNAL OF BIOCHEMISTRY, 35(1), 16-21.

Bulk preparation of hepatic binding protein

PESSINA, G. P.;NALDINI, A.;BOCCI, V.
1986

Abstract

A method for the extraction in the cold of hepatic binding protein using a novel combination of detergents, namely 1% Na urso deoxycholate and 5% Tween 80 in the extraction buffer, which allows a significantly higher recovery of lectin than 1% Triton X-100 is described. It is noteworthy that this lectin preparation can be maintained in solution for over a month and can be used in vitro and in vivo.
Pessina, G.P., Naldini, A., & Bocci, V. (1986). Bulk preparation of hepatic binding protein. ITALIAN JOURNAL OF BIOCHEMISTRY, 35(1), 16-21.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/8163
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo