Resolution enhancement of nitrogen hyperfine patterns in the EPR spectra of Cu(II) complexes: FT analysis of Cu(II)(His-Gly)2

Fourier analysis of EPR spectra is used to achieve straightforward discrimination between an even or odd number of nitrogen nuclei bound to copper in solution. The method is rested on a copper (II) dipeptide (Cu-HistidylGlycine) complex to confirm the room temperature structural arrangement previously hypothesized by conventional techniques. Any residual uncertainty about ligand coordination around copper in solution is eliminated by the present approach. This simple straightforward method is particularly useful for extracting information from poorly resolved patterns of room temperature EPR spectra.