OBJECTIVE: To measure activin A concentrations in uterine washing fluid collected from women with couple infertility undergoing intrauterine insemination (IUI). DESIGN: Retrospective case-control study. SETTING: Tertiary university center for women's care. PATIENT(S): Fifty women, of whom 25 became pregnant after up to three IUI attempts and 25 did not. INTERVENTION(S): Endocrine and clinical evaluation, semen analyses and hypo-osmotic swelling test, ovarian stimulation, endometrial thickness measurement by ultrasound, uterine washing fluid collection by sonohysterography, and IUI. MAIN OUTCOME MEASURE(S): Activin A measurement by enzyme-linked immunosorbent assay, receiver operating characteristics curve analysis, and pregnancy rates after IUI; sensitivity, specificity, positive and negative likelihood ratios of activin A, and endometrial thickness for pregnancy prediction. RESULT(S): Activin A was measurable in all samples evaluated, and the levels (mean +/- SEM) were statistically significantly higher in the pregnant (0.08 +/- 0.01 ng/mL) than in the nonpregnant (0.022 +/- 0.001 ng/mL) group. Activin A at the cut off of 0.04 ng/mL achieved a sensitivity of 76.0 % (95% CI, 54.9%-90.6%) and a specificity of 100% (95% CI, 86.2%-100%) as a single marker for prediction of pregnancy. CONCLUSION(S): Activin A is secreted into uterine lumen; the levels in endometrial washing fluids were higher in women who subsequently became pregnant after IUI, so its measurement may be useful in predicting successful implantation

Florio, P., Bruni, L., Galleri, L., Reis, F.M., Borges, L.E., Bocchi, C., et al. (2010). Evaluation of endometrial activin A secretion for prediction of pregnancy after intrauterine insemination. FERTILITY AND STERILITY, 93(7), 2316-2320 [10.1016/j.fertnstert.2008.12.125].

Evaluation of endometrial activin A secretion for prediction of pregnancy after intrauterine insemination

FLORIO, P.;BRUNI, L.;GALLERI, L.;BOCCHI, C.;DE LEO, V.;
2010

Abstract

OBJECTIVE: To measure activin A concentrations in uterine washing fluid collected from women with couple infertility undergoing intrauterine insemination (IUI). DESIGN: Retrospective case-control study. SETTING: Tertiary university center for women's care. PATIENT(S): Fifty women, of whom 25 became pregnant after up to three IUI attempts and 25 did not. INTERVENTION(S): Endocrine and clinical evaluation, semen analyses and hypo-osmotic swelling test, ovarian stimulation, endometrial thickness measurement by ultrasound, uterine washing fluid collection by sonohysterography, and IUI. MAIN OUTCOME MEASURE(S): Activin A measurement by enzyme-linked immunosorbent assay, receiver operating characteristics curve analysis, and pregnancy rates after IUI; sensitivity, specificity, positive and negative likelihood ratios of activin A, and endometrial thickness for pregnancy prediction. RESULT(S): Activin A was measurable in all samples evaluated, and the levels (mean +/- SEM) were statistically significantly higher in the pregnant (0.08 +/- 0.01 ng/mL) than in the nonpregnant (0.022 +/- 0.001 ng/mL) group. Activin A at the cut off of 0.04 ng/mL achieved a sensitivity of 76.0 % (95% CI, 54.9%-90.6%) and a specificity of 100% (95% CI, 86.2%-100%) as a single marker for prediction of pregnancy. CONCLUSION(S): Activin A is secreted into uterine lumen; the levels in endometrial washing fluids were higher in women who subsequently became pregnant after IUI, so its measurement may be useful in predicting successful implantation
Florio, P., Bruni, L., Galleri, L., Reis, F.M., Borges, L.E., Bocchi, C., et al. (2010). Evaluation of endometrial activin A secretion for prediction of pregnancy after intrauterine insemination. FERTILITY AND STERILITY, 93(7), 2316-2320 [10.1016/j.fertnstert.2008.12.125].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/6495
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