Morphine, codeine, morphine-6-glucuronide, and morphine-3-glucuronide are synthesized de novo in mammalian cells and in the central nervous system. Knowledge on endogenous morphine-like compound distribution in the adult mouse brain has been recently improved, and new hypotheses have been suggested about the potential implications in brain physiology. Endogenous morphine-like compounds have been shown to be synthesized in the spinal cord, but their localization is unknown. Here we describe the distribution of endogenous morphine-like compounds (morphine and/or its glucuronides and/or codeine) in the adult mouse spinal cord using a well-validated antibody. By using different microscopy approaches, we found the presence of morphine, codeine, or morphine glucuronides in ?-aminobutyric acid (GABA)-ergic neurons and astrocytes of the spinal cord. Whereas GABAergic neurons containing endogenous morphine-like compounds were located primarily in the ventral horn, astrocytes that were labeled for morphine-like compounds were found throughout the gray matter and the white matter. Our study demonstrates the possibility that endogenous morphine-like compounds in the central nervous system have other functions beyond their analgesic functions. J. Comp. Neurol. 520:15471561, 2012. (c) 2011 Wiley Periodicals, Inc.

A., L., F., D., J., M., D., S., A., D., Bianchi, E., et al. (2012). Localization of endogenous morphine-like compounds in the mouse spinal cord. JOURNAL OF COMPARATIVE NEUROLOGY, 520(7), 1547-1561 [10.1002/cne.22811].

Localization of endogenous morphine-like compounds in the mouse spinal cord

BIANCHI, ENRICA;
2012

Abstract

Morphine, codeine, morphine-6-glucuronide, and morphine-3-glucuronide are synthesized de novo in mammalian cells and in the central nervous system. Knowledge on endogenous morphine-like compound distribution in the adult mouse brain has been recently improved, and new hypotheses have been suggested about the potential implications in brain physiology. Endogenous morphine-like compounds have been shown to be synthesized in the spinal cord, but their localization is unknown. Here we describe the distribution of endogenous morphine-like compounds (morphine and/or its glucuronides and/or codeine) in the adult mouse spinal cord using a well-validated antibody. By using different microscopy approaches, we found the presence of morphine, codeine, or morphine glucuronides in ?-aminobutyric acid (GABA)-ergic neurons and astrocytes of the spinal cord. Whereas GABAergic neurons containing endogenous morphine-like compounds were located primarily in the ventral horn, astrocytes that were labeled for morphine-like compounds were found throughout the gray matter and the white matter. Our study demonstrates the possibility that endogenous morphine-like compounds in the central nervous system have other functions beyond their analgesic functions. J. Comp. Neurol. 520:15471561, 2012. (c) 2011 Wiley Periodicals, Inc.
A., L., F., D., J., M., D., S., A., D., Bianchi, E., et al. (2012). Localization of endogenous morphine-like compounds in the mouse spinal cord. JOURNAL OF COMPARATIVE NEUROLOGY, 520(7), 1547-1561 [10.1002/cne.22811].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/500766
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