Experiments assessed whether long term exposure to 50 Hz pulsed electromagnetic fields with a peak magnetic field of 3 mT can alter the dynamics of intracellular calcium in human astrocytoma U-373 MG cells. Pretreatment of cells with 1.2 μM substance P significantly increased the [Ca2+]i. The same effect was also observed when [Ca2+]i was evaluated in the presence of 20 mM caffeine. After exposure to electromagnetic fields the basal [Ca2+]i levels increased significantly from 143 ± 46 nM to 278 ± 125 nM. The increase was also evident after caffeine addition, but in cells treated with substance P and substance P + caffeine we observed a [Ca2+]i decrease after exposure. When we substituted calcium-free medium for normal medium immediately before the [Ca2+]i measurements, the [Ca2+]i was similar to that measured in the presence of Ca2+. In this case, after EMFs exposure of cells treated with substance P, the [Ca2+]i, measured without and with addition of caffeine, declined from 824 ± 425 to 38 ± 13 nM and from 1369 ± 700 to 11 ± 4 nM, respectively, indicating that electromagnetic fields act either on intracellular Ca2+ stores or on the plasma membrane. Moreover the electromagnetic fields that affected [Ca2+]i did not cause cell proliferation or cell death and the proliferation indexes remained unchanged after exposure. © 2001 Wiley-Liss, Inc.

Pessina, G.P., Aldinucci, C., Palmi, M., Sgaragli, G.P., Benocci, A., Pessina, F. (2001). Pulsed Electromognetic field affects the intracellular calcium concentration in human astrocytoma cells. BIOELECTROMAGNETICS, 22(7), 503-510 [10.1002/bem.79].

Pulsed Electromognetic field affects the intracellular calcium concentration in human astrocytoma cells

PESSINA, G. P.;ALDINUCCI, C.;PALMI, M.;SGARAGLI, G. P.;PESSINA, F.
2001-01-01

Abstract

Experiments assessed whether long term exposure to 50 Hz pulsed electromagnetic fields with a peak magnetic field of 3 mT can alter the dynamics of intracellular calcium in human astrocytoma U-373 MG cells. Pretreatment of cells with 1.2 μM substance P significantly increased the [Ca2+]i. The same effect was also observed when [Ca2+]i was evaluated in the presence of 20 mM caffeine. After exposure to electromagnetic fields the basal [Ca2+]i levels increased significantly from 143 ± 46 nM to 278 ± 125 nM. The increase was also evident after caffeine addition, but in cells treated with substance P and substance P + caffeine we observed a [Ca2+]i decrease after exposure. When we substituted calcium-free medium for normal medium immediately before the [Ca2+]i measurements, the [Ca2+]i was similar to that measured in the presence of Ca2+. In this case, after EMFs exposure of cells treated with substance P, the [Ca2+]i, measured without and with addition of caffeine, declined from 824 ± 425 to 38 ± 13 nM and from 1369 ± 700 to 11 ± 4 nM, respectively, indicating that electromagnetic fields act either on intracellular Ca2+ stores or on the plasma membrane. Moreover the electromagnetic fields that affected [Ca2+]i did not cause cell proliferation or cell death and the proliferation indexes remained unchanged after exposure. © 2001 Wiley-Liss, Inc.
2001
Pessina, G.P., Aldinucci, C., Palmi, M., Sgaragli, G.P., Benocci, A., Pessina, F. (2001). Pulsed Electromognetic field affects the intracellular calcium concentration in human astrocytoma cells. BIOELECTROMAGNETICS, 22(7), 503-510 [10.1002/bem.79].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/49997
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