During our researches on systematics and ecology of terrestrial isopods, carried out in western Sicily, some specimens showing a blue-purple coloration were collected; they belonged to four species: Armadillidium decorum Brandt, 1833, Trichoniscus panormidensis Montesanto et al., 2011, Philoscia affinis Verhoeff, 1908, Porcellio siculoccidentalis Viglianisi et al., 1992. We hypothesized that such coloration could be due, as reported in literature, to characteristic paracrystalline arrays of virions inside the tissues of blue colored specimens. Ultrastructural observations by transmission electron microscopy, on tissues of A. decorum, showed the presence of electron-dense viral particles, with a diameter of nearly 0.12 mu m. Dual-axis tomography, performed on specimens of A. decorum, evidenced an icosahedral structure of viral particles matching with that of Isopod Iridescent Virus (IIV). Molecular analysis, on 254 bp portion of the major capsid protein (MCP) gene, allowed to place the virus into IIV-31 group, already known for other oniscidean species. The symptoms of infected individuals and the courseof the disease were followed in laboratory, indicating similarities with other studies on Isopod Iridoviruses. Moreover, some notes on reproduction of infected ovigerous females are reported. Our data support unequivocal and direct evidences for the first case of IIV infection in terrestrial isopods reported in Italy.

Lupetti, P., Montesanto, G., Ciolfi, S., Marri, L., Gentile, M., Paccagnini, E., et al. (2013). Iridovirus infection in terrestrial isopods from Sicily (Italy). TISSUE & CELL, 45(5), 321-327 [10.1016/j.tice.2013.05.001].

Iridovirus infection in terrestrial isopods from Sicily (Italy)

LUPETTI, PIETRO;CIOLFI, SILVIA;MARRI, LAURA;GENTILE M.;PACCAGNINI, EUGENIO;
2013-01-01

Abstract

During our researches on systematics and ecology of terrestrial isopods, carried out in western Sicily, some specimens showing a blue-purple coloration were collected; they belonged to four species: Armadillidium decorum Brandt, 1833, Trichoniscus panormidensis Montesanto et al., 2011, Philoscia affinis Verhoeff, 1908, Porcellio siculoccidentalis Viglianisi et al., 1992. We hypothesized that such coloration could be due, as reported in literature, to characteristic paracrystalline arrays of virions inside the tissues of blue colored specimens. Ultrastructural observations by transmission electron microscopy, on tissues of A. decorum, showed the presence of electron-dense viral particles, with a diameter of nearly 0.12 mu m. Dual-axis tomography, performed on specimens of A. decorum, evidenced an icosahedral structure of viral particles matching with that of Isopod Iridescent Virus (IIV). Molecular analysis, on 254 bp portion of the major capsid protein (MCP) gene, allowed to place the virus into IIV-31 group, already known for other oniscidean species. The symptoms of infected individuals and the courseof the disease were followed in laboratory, indicating similarities with other studies on Isopod Iridoviruses. Moreover, some notes on reproduction of infected ovigerous females are reported. Our data support unequivocal and direct evidences for the first case of IIV infection in terrestrial isopods reported in Italy.
Lupetti, P., Montesanto, G., Ciolfi, S., Marri, L., Gentile, M., Paccagnini, E., et al. (2013). Iridovirus infection in terrestrial isopods from Sicily (Italy). TISSUE & CELL, 45(5), 321-327 [10.1016/j.tice.2013.05.001].
File in questo prodotto:
File Dimensione Formato  
Tissue and Cell_2013.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 3.45 MB
Formato Adobe PDF
3.45 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/46480
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo