Ozonated oils as functional dermatological matrices: Effects on the wound healing process using SKH1 mice

Wound tissue repair is a complex and dynamic process of restoring cellular structures and tissue layers.Improvement of this process is crucial for several pathologies characterized by chronic delayed woundclosure such as diabetes, and the investigation of new approaches aimed to ameliorate the wound healingprocess is under continuous evolution. Recently, the usage of vegetable matrices in the form of ozonatedoils has been proposed and several researchers have shown a positive effect in the wound, based on theirbactericidal, antiviral, and antifungal properties. The present study was undertaken to compare the effectthat different ozonated oils (olive, sesame and linseed) with the same level of ozonation have on woundhealing rate in SKH1 mice. Several histological parameters and the level of key proteins such as VEGF andPCNA have been analyzed. Only treatment with ozonated sesame oil shows a faster wound closure in thefirst 7 days. This effect paralleled with the increased VEGF and PCNA levels, NFkB nuclear translocationand 4-HNE formation. The present study shows that not only the ozonation grade is of importance for the improvement of wound healing process but also the typical composition of the oil.