Objectives  To investigate the effect of St John's wort (SJW) and its active constituents hypericin and hyperforin on detrusor smooth muscle contractility and their possible neuroprotective role against ischaemic-like conditions, which could arise during overactive bladder disease. Methods  In whole bladders, intrinsic nerves underwent electrical field stimulation (EFS). The effect of drugs on the contractile response and its recovery in reperfusion phase (R) was monitored at different concentrations during 1 or 2 h of anoxia-glucopenia (A-G) and the first 30 min of R. The effects of the drugs were also investigated on rat detrusor muscle strips contracted with carbachol, KCl and electrically. Key Findings  SJW has spasmolytic activity, which increases with increasing concentration and it worsens the damage induced by A-G/R on rat urinary bladder. Hypericin and hyperforin had no effect during ischemic-like conditions but they both exert a dual modulation of rat detrusor strips contraction. At high micromolar concentrations they showed a relaxing effect, but at submicromolar range hypericin increased the plasma membrane depolarisation and hyperforin showed a stimulatory effect on the cholinergic system. Conclusions  The results of our study showed that SJW and its constituents could modulate urinary bladder contractility and even worsen A-G/R injury.

Valeri, A., Capasso, R., Valoti, M., Pessina, F. (2012). Effects of St John's wort and its active constituents, hypericin and hyperforin, on isolated rat urinary bladder. JOURNAL OF PHARMACY AND PHARMACOLOGY, 64(12), 1770-1776 [10.1111/j.2042-7158.2012.01556.x].

Effects of St John's wort and its active constituents, hypericin and hyperforin, on isolated rat urinary bladder

Valeri, A.;Valoti, M.;Pessina, F.
2012-01-01

Abstract

Objectives  To investigate the effect of St John's wort (SJW) and its active constituents hypericin and hyperforin on detrusor smooth muscle contractility and their possible neuroprotective role against ischaemic-like conditions, which could arise during overactive bladder disease. Methods  In whole bladders, intrinsic nerves underwent electrical field stimulation (EFS). The effect of drugs on the contractile response and its recovery in reperfusion phase (R) was monitored at different concentrations during 1 or 2 h of anoxia-glucopenia (A-G) and the first 30 min of R. The effects of the drugs were also investigated on rat detrusor muscle strips contracted with carbachol, KCl and electrically. Key Findings  SJW has spasmolytic activity, which increases with increasing concentration and it worsens the damage induced by A-G/R on rat urinary bladder. Hypericin and hyperforin had no effect during ischemic-like conditions but they both exert a dual modulation of rat detrusor strips contraction. At high micromolar concentrations they showed a relaxing effect, but at submicromolar range hypericin increased the plasma membrane depolarisation and hyperforin showed a stimulatory effect on the cholinergic system. Conclusions  The results of our study showed that SJW and its constituents could modulate urinary bladder contractility and even worsen A-G/R injury.
2012
Valeri, A., Capasso, R., Valoti, M., Pessina, F. (2012). Effects of St John's wort and its active constituents, hypericin and hyperforin, on isolated rat urinary bladder. JOURNAL OF PHARMACY AND PHARMACOLOGY, 64(12), 1770-1776 [10.1111/j.2042-7158.2012.01556.x].
File in questo prodotto:
File Dimensione Formato  
JPP.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 512.41 kB
Formato Adobe PDF
512.41 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/44271
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo