During seed formation of Brassica macrocarpa the development of the embryo precedes that of the integuments; structural changes and histochemical changes are associated. Esterases, acid phosphatases, phenols and starch follow a sigmoid pattern, increasing during embryogenesis and decreasing during seed maturation. In the mature seed, esterase activity is localized in the embryo and in the cells of the mucilaginous, aleuronic and hyaline layers. Acid phosphatases are present in the mucilaginous cells, mainly in the column, the cell walls delimiting intercellular spaces of the cortical cylinder and the adhesion areas of the cotyledons. Phenols are scanty in the root apex, mucilaginous cells and the palisade layer, and abundant in the pigmented layer. Starch is absent in ripe seeds which have lipid and protein reserves. The major classes of storage proteins have molecular weights of 21, 22, 27 and 30 KD and accumulate in the late stages prior to complete drying. Esterases and acid phosphatases in mucilaginous cells of the seed integument suggest that these enzymes are involved in hydrolytic processes occurring prior to germination and that mucilages have a metabolic function in seed-soil interactions.
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|Titolo:||Structural and histochemical changes during seed development of Brassica macrocarpa Guss|
|Citazione:||Scialabba, A., DI LIBERTO, C., Bellani, L., & Muccifora, S. (1999). Structural and histochemical changes during seed development of Brassica macrocarpa Guss. EUROPEAN JOURNAL OF HISTOCHEMISTRY, 43, 147-154.|
|Appare nelle tipologie:||1.1 Articolo in rivista|