Rat cerebrum microsomes were subfractionated on isopycnic linear sucrose (20-42%) density gradients. The Ca2+ loading/release properties and the distribution of intracellular Ca2+ store channels, inositol 1,4,5-trisphosphate (IP3) receptor and ryanodine (Ry) receptor, and SERCA pumps, were monitored in each subfraction by ligand binding and 45Ca2+ loading/release assays. Three different classes of vesicles were identified: (i) heavy density vesicles with high content of Ry receptors and Ca2+ pumps and high thapsigargin (TG)-sensitivity of Ca2+ loading; (ii) intermediate sucrose density vesicles with high content of IP3 receptor, high IP(S)3-sensitivity of Ca2+ loading and low content of Ry receptors; and (iii) light sucrose density vesicles with high content of Ry receptors, low content of IP3 receptors and low content of SERCA pumps highly sensitive to TG. Isolation of molecularly heterogeneous rat cerebrum microsomes and identification of specific Ca2+ loading/release properties support the presence of multiple, potentially active, heterogeneous rapidly exchanging Ca2+ stores in rat cerebrum.

Nori, A., Fulceri, R., Gamberucci, A., Benedetti, A., & Volpe, P. (1996). Biochemical and functional heterogenity of rat cerebrum microsomal membranes in relation to SERCA Ca2+-ATPases and Ca2+ release channels. CELL CALCIUM, 19(5), 375-381 [10.1016/S0143-4160(96)90110-4].

Biochemical and functional heterogenity of rat cerebrum microsomal membranes in relation to SERCA Ca2+-ATPases and Ca2+ release channels

Fulceri, R.;Gamberucci, Alessandra;Benedetti, Angiolo;
1996

Abstract

Rat cerebrum microsomes were subfractionated on isopycnic linear sucrose (20-42%) density gradients. The Ca2+ loading/release properties and the distribution of intracellular Ca2+ store channels, inositol 1,4,5-trisphosphate (IP3) receptor and ryanodine (Ry) receptor, and SERCA pumps, were monitored in each subfraction by ligand binding and 45Ca2+ loading/release assays. Three different classes of vesicles were identified: (i) heavy density vesicles with high content of Ry receptors and Ca2+ pumps and high thapsigargin (TG)-sensitivity of Ca2+ loading; (ii) intermediate sucrose density vesicles with high content of IP3 receptor, high IP(S)3-sensitivity of Ca2+ loading and low content of Ry receptors; and (iii) light sucrose density vesicles with high content of Ry receptors, low content of IP3 receptors and low content of SERCA pumps highly sensitive to TG. Isolation of molecularly heterogeneous rat cerebrum microsomes and identification of specific Ca2+ loading/release properties support the presence of multiple, potentially active, heterogeneous rapidly exchanging Ca2+ stores in rat cerebrum.
Nori, A., Fulceri, R., Gamberucci, A., Benedetti, A., & Volpe, P. (1996). Biochemical and functional heterogenity of rat cerebrum microsomal membranes in relation to SERCA Ca2+-ATPases and Ca2+ release channels. CELL CALCIUM, 19(5), 375-381 [10.1016/S0143-4160(96)90110-4].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/438483