Menadione is selectively toxic to erythrocytes. Although GSH is considered a primary target of menadione, intraerythrocyte thiolic alterations consequent to menadione exposure are only partially known. In this study alterations of GSH and protein thiols (PSH) and their relationship with methemoglobin formation were investigated in human and rat red blood cells (RBC) exposed to menadione. In both erythrocyte types, menadione caused a marked increase in methemoglobin associated with GSH depletion and increased oxygen consumption. However, in human RBC, GSH formed a conjugate with menadione, whereas, in rat RBC it was converted to GSSG, concomitantly with a loss of protein thiols (corresponding to menadione arylation), and an increase in glutathione-protein mixed disulfides (GS-SP). Such differences were related to the presence of highly reactive cysteines, which characterize rat hemoglobin (cys beta125). In spite of the greater thiol oxidation in rat than in human RBC, methemoglobin formation and the rate of oxygen consumption elicited by menadione in both species were rather similar. Moreover, in repeated experiments under N2 or CO-blocked heme, it was found that menadione conjugation (arylation) in both species was riot dependent oil the presence of oxygen or the status of heme. Therefore, we assumed that GSH (human RBC) and protein (rat RBC) arylation was equally responsible for increased oxygen consumption and Hb oxidation. Moreover, thiol oxidation of rat RBC was strictly related to methemoglobin formation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Lusini, L., Rossi, R., Giustarini, D., Di Simplicio, P. (2002). The pro-oxidant role of protein SH groups of haemoglobin in rat erythrocytes exposed to menadione. CHEMICO-BIOLOGICAL INTERACTIONS, 139(1), 97-114 [10.1016/S0009-2797(01)00296-4].

The pro-oxidant role of protein SH groups of haemoglobin in rat erythrocytes exposed to menadione

ROSSI, RANIERI;GIUSTARINI, DANIELA;
2002-01-01

Abstract

Menadione is selectively toxic to erythrocytes. Although GSH is considered a primary target of menadione, intraerythrocyte thiolic alterations consequent to menadione exposure are only partially known. In this study alterations of GSH and protein thiols (PSH) and their relationship with methemoglobin formation were investigated in human and rat red blood cells (RBC) exposed to menadione. In both erythrocyte types, menadione caused a marked increase in methemoglobin associated with GSH depletion and increased oxygen consumption. However, in human RBC, GSH formed a conjugate with menadione, whereas, in rat RBC it was converted to GSSG, concomitantly with a loss of protein thiols (corresponding to menadione arylation), and an increase in glutathione-protein mixed disulfides (GS-SP). Such differences were related to the presence of highly reactive cysteines, which characterize rat hemoglobin (cys beta125). In spite of the greater thiol oxidation in rat than in human RBC, methemoglobin formation and the rate of oxygen consumption elicited by menadione in both species were rather similar. Moreover, in repeated experiments under N2 or CO-blocked heme, it was found that menadione conjugation (arylation) in both species was riot dependent oil the presence of oxygen or the status of heme. Therefore, we assumed that GSH (human RBC) and protein (rat RBC) arylation was equally responsible for increased oxygen consumption and Hb oxidation. Moreover, thiol oxidation of rat RBC was strictly related to methemoglobin formation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
2002
Lusini, L., Rossi, R., Giustarini, D., Di Simplicio, P. (2002). The pro-oxidant role of protein SH groups of haemoglobin in rat erythrocytes exposed to menadione. CHEMICO-BIOLOGICAL INTERACTIONS, 139(1), 97-114 [10.1016/S0009-2797(01)00296-4].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/43333
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