The behaviour of 5'-nucleotidase isoenzymes (ecto-5'-nucleotidase, e-Ns and c-N-II soluble 5'-nucleotidases) was studied in lymphocytes from patients with B-cell chronic lymphocytic leukemia. A strong reduction in ecto- and soluble activities was observed, although the pattern of the three 5'-nucleotidases did not always strictly overlap. A significant decrease (p<0.05) in ecto-5'-nucleotidase, e-Ns and c-N-II was found in B and T populations (B lymphocytes: 1.13, 0.88 and 1.26 nmol/h/10(6) cells versus 95.96, 9.64 and 13.73 nmol/h/10(6) cells in controls; T lymphocytes: 1.31, 0.23 and 0.06 nmol/h/10(6) cells versus 9.25, 1.31 and 2.10 nmol/h/10(6) cells in healthy subjects). The percentage of ecto-5'-nucleotidase-positive cells (CD73+) was reduced in leukemia patients, indicating a lower number of active molecules on the cell surface. The results of RT-PCR analysis showed that the ecto-5'-nucleotidase mRNA of leukemia patients was not defective

Rosi, F., Agostinho, A.B., Carlucci, F., Zanoni, L., Porcelli, B., Marinello, E., et al. (1998). Behaviour of human lymphocytic isoenzymes of 5' nucleotidase. LIFE SCIENCES, 62(25), 2257-2266 [10.1016/S0024-3205(98)00206-9].

Behaviour of human lymphocytic isoenzymes of 5' nucleotidase

CARLUCCI F.;PORCELLI B.;MARINELLO E.;TABUCCHI A.
1998-01-01

Abstract

The behaviour of 5'-nucleotidase isoenzymes (ecto-5'-nucleotidase, e-Ns and c-N-II soluble 5'-nucleotidases) was studied in lymphocytes from patients with B-cell chronic lymphocytic leukemia. A strong reduction in ecto- and soluble activities was observed, although the pattern of the three 5'-nucleotidases did not always strictly overlap. A significant decrease (p<0.05) in ecto-5'-nucleotidase, e-Ns and c-N-II was found in B and T populations (B lymphocytes: 1.13, 0.88 and 1.26 nmol/h/10(6) cells versus 95.96, 9.64 and 13.73 nmol/h/10(6) cells in controls; T lymphocytes: 1.31, 0.23 and 0.06 nmol/h/10(6) cells versus 9.25, 1.31 and 2.10 nmol/h/10(6) cells in healthy subjects). The percentage of ecto-5'-nucleotidase-positive cells (CD73+) was reduced in leukemia patients, indicating a lower number of active molecules on the cell surface. The results of RT-PCR analysis showed that the ecto-5'-nucleotidase mRNA of leukemia patients was not defective
1998
Rosi, F., Agostinho, A.B., Carlucci, F., Zanoni, L., Porcelli, B., Marinello, E., et al. (1998). Behaviour of human lymphocytic isoenzymes of 5' nucleotidase. LIFE SCIENCES, 62(25), 2257-2266 [10.1016/S0024-3205(98)00206-9].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/42656
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