Purpose. To determine the microbial contamination of the irrigating fluids at the time of phacoemulsification after the use of topical povidone-iodine and antibiotics prophylaxis. Methods. A total of 119 patients undergoing cataract surgery were enrolled in this prospective study. All patients received 5 mg/mL levofloxacin starting from the day prior to surgery and topical and 5% povidone-iodine drops starting from 30 minutes before the surgery. At the end of each surgery, 2 samples of drainage liquids were sterilely collected from the drainage bags (DBL) and from the peristaltic pump single-cassettes (PCL) of the phacoemulsification machine. Search for aerobic and anaerobic bacteria and fungi was performed. Results. Seventy-five patients (31.5%) revealed a growth of at least one microbial species (53 DBL and 22 PCL, 44.5% vs 18.5%; p<0.001). Sixty-six patients (55.5%) had at least one positive intraoperative solution. Overall, 111 microbial strains were collected: 82 (74%) Gram-positive bacteria, 20 (18%) fungi, and 9 (8%) Gram-negative bacteria. Thirteen staphylococcal isolates from PCL, compared with 52 out of DBL (11% vs 43.7%, p<0.001), fungi were essentially isolated from PCL. No significant correlation was found between microbial isolation and risk factors. No postsurgical infective complication occurred in the follow-up. Conclusions. Evaluation of intraoperative fluids can provide evidence on sources or vehicles of postsurgical infections. Antibiotic prophylaxis and topical povidone-iodine can significantly contribute to minimize the risk of endophthalmitis.

Balestrazzi, A., Malandrini, A., Montagnani, F., Nguisseu Chegoua, G.L., Ciompi, L., Zanchi, A., et al. (2012). Phacoemulsificator and sterile drapes contamination during cataract surgery: a microbiological study. EUROPEAN JOURNAL OF OPHTHALMOLOGY, 22(2), 188-194 [10.5301/EJO.2011.8434].

Phacoemulsificator and sterile drapes contamination during cataract surgery: a microbiological study.

BALESTRAZZI, ANGELO;MALANDRINI, ALEX;MONTAGNANI, FRANCESCA;CIOMPI, LEONARDO;TOSI, GIAN MARCO;MOTOLESE, ILARIA;FRUSCHELLI, MARIO
2012-01-01

Abstract

Purpose. To determine the microbial contamination of the irrigating fluids at the time of phacoemulsification after the use of topical povidone-iodine and antibiotics prophylaxis. Methods. A total of 119 patients undergoing cataract surgery were enrolled in this prospective study. All patients received 5 mg/mL levofloxacin starting from the day prior to surgery and topical and 5% povidone-iodine drops starting from 30 minutes before the surgery. At the end of each surgery, 2 samples of drainage liquids were sterilely collected from the drainage bags (DBL) and from the peristaltic pump single-cassettes (PCL) of the phacoemulsification machine. Search for aerobic and anaerobic bacteria and fungi was performed. Results. Seventy-five patients (31.5%) revealed a growth of at least one microbial species (53 DBL and 22 PCL, 44.5% vs 18.5%; p<0.001). Sixty-six patients (55.5%) had at least one positive intraoperative solution. Overall, 111 microbial strains were collected: 82 (74%) Gram-positive bacteria, 20 (18%) fungi, and 9 (8%) Gram-negative bacteria. Thirteen staphylococcal isolates from PCL, compared with 52 out of DBL (11% vs 43.7%, p<0.001), fungi were essentially isolated from PCL. No significant correlation was found between microbial isolation and risk factors. No postsurgical infective complication occurred in the follow-up. Conclusions. Evaluation of intraoperative fluids can provide evidence on sources or vehicles of postsurgical infections. Antibiotic prophylaxis and topical povidone-iodine can significantly contribute to minimize the risk of endophthalmitis.
2012
Balestrazzi, A., Malandrini, A., Montagnani, F., Nguisseu Chegoua, G.L., Ciompi, L., Zanchi, A., et al. (2012). Phacoemulsificator and sterile drapes contamination during cataract surgery: a microbiological study. EUROPEAN JOURNAL OF OPHTHALMOLOGY, 22(2), 188-194 [10.5301/EJO.2011.8434].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/41627
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