In this work, protein-glutathione mixed disulfide formation in human red blood cells (RBCs) was evaluated in vitro by using the thiol-specific reagent diamide. We investigated what mechanism could lead to S-glutathionylation of membrane skeletal proteins, what are the main target proteins, and the correlation between protein S-glutathionylation and RBC hemolysis. Diamide caused a decrease in the reduced form of glutathione (GSH), which was accompanied by an increase in the basal level of glutathione disulfide (GSSG) and in S-glutathionylation of protein 4.2 and spectrin. The increase in membrane skeletal protein S-glutathionylation was correlated with a lower susceptibility of RBCs to osmotic hemolysis, suggesting that S-glutathionylation of protein 4.2 and spectrin could contribute to regulate RBC membrane stability.

Rossi, R., Giustarini, D., Milzani, A., & AND DALLE DONNE, I. (2006). Membrane skeletal protein S-glutathionylation and hemolysis in human red blood cells. BLOOD CELLS, MOLECULES, & DISEASES, 37(3), 180-187 [10.1016/j.bcmd.2006.09.003].

Membrane skeletal protein S-glutathionylation and hemolysis in human red blood cells

ROSSI, RANIERI;GIUSTARINI D;
2006

Abstract

In this work, protein-glutathione mixed disulfide formation in human red blood cells (RBCs) was evaluated in vitro by using the thiol-specific reagent diamide. We investigated what mechanism could lead to S-glutathionylation of membrane skeletal proteins, what are the main target proteins, and the correlation between protein S-glutathionylation and RBC hemolysis. Diamide caused a decrease in the reduced form of glutathione (GSH), which was accompanied by an increase in the basal level of glutathione disulfide (GSSG) and in S-glutathionylation of protein 4.2 and spectrin. The increase in membrane skeletal protein S-glutathionylation was correlated with a lower susceptibility of RBCs to osmotic hemolysis, suggesting that S-glutathionylation of protein 4.2 and spectrin could contribute to regulate RBC membrane stability.
Rossi, R., Giustarini, D., Milzani, A., & AND DALLE DONNE, I. (2006). Membrane skeletal protein S-glutathionylation and hemolysis in human red blood cells. BLOOD CELLS, MOLECULES, & DISEASES, 37(3), 180-187 [10.1016/j.bcmd.2006.09.003].
File in questo prodotto:
File Dimensione Formato  
2006 Rossi BCMD.pdf

non disponibili

Descrizione: Author's copy
Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 434.16 kB
Formato Adobe PDF
434.16 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Membrane skeletal protein S-glutathionylation and hemolysis in human red blood cells.pdf

non disponibili

Descrizione: definitivo
Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 328.88 kB
Formato Adobe PDF
328.88 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/412367