Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.

Giustarini, D., Milzani, A., DALLE DONNE, I., & Rossi, R. (2007). Detection of S-nitrosothiols in biological fluids: a comparison among the most widely applied methodologies. JOURNAL OF CHROMATOGRAPHY. B, 851(1-2), 124-139 [10.1016/j.jchromb.2006.09.031].

Detection of S-nitrosothiols in biological fluids: a comparison among the most widely applied methodologies

GIUSTARINI D;ROSSI, RANIERI
2007

Abstract

Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.
Giustarini, D., Milzani, A., DALLE DONNE, I., & Rossi, R. (2007). Detection of S-nitrosothiols in biological fluids: a comparison among the most widely applied methodologies. JOURNAL OF CHROMATOGRAPHY. B, 851(1-2), 124-139 [10.1016/j.jchromb.2006.09.031].
File in questo prodotto:
File Dimensione Formato  
2007 Giustarini JCB.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 348.84 kB
Formato Adobe PDF
348.84 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/412308