Immobilization of Heparin and Highly-Sulphated Hyaluronic Acid onto Plasma-Treated Polyethylene

Heparin and highly-sulphated hyaluronic acid have been successfully immobilized onto plasma-processed polyethylene via a diamine polyethyleneglycol (PEG) spacer molecule. Two different plasma-processes have been utilized, i.e. a treatment and a deposition process, for providing polyethylene surface with the -COOH groups necessary for the immobilization reactions. XPS integrated with derivatization procedures, ATR-FTIR and Water Contact Angle measurements have been carried out for characterizing each modification step: 1) the plasmaprocess, 2) the immobilization of the spacer molecule and 3) the immobilization of the biomolecules. The thrombin time of the modified surfaces has been measured, and their platelet activation characteristics evaluated. The results indicate a certain nonthrombogenic character of the biomolecule-immobilized polyethylene samples. © 1998 Plenum Publishing Corporation.