The possibility that certain Mediterranean cetaceans are subject to toxicological hazard due to organochlorines and emerging contaminants, such as polybrominated diphenyl ethers (PBDEs) with endocrine disrupting capacity, was investigated using non-lethal methods. The need for new biomarkers for EDCs and for a “cell model” to explore the diVerent susceptibilities to several classes of ECDs, including emerging contaminants, led us to culture Wbroblasts of diVerent cetacean species (“dolphins in test tubes”). We then explored interspecies and gender susceptibility to OC-EDCs and PBDEs using qualitative and semi-quantitative evaluation of target proteins, such as CYP1A and CYP2B in cultured cetacean Wbroblasts (Stenella coeruleoalba, Tursiops truncatus and Balaenoptera physalus), by western blot and immunoXuorescence techniques.
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|Titolo:||Development of new-tools to investigate toxicological hazard due to endocrine disruptor organochlorines and emerging contaminants in mediterranean cetaceans|
|Citazione:||Fossi, M.C., Marsili, L., Casini, S., & Bucalossi, D. (2006). Development of new-tools to investigate toxicological hazard due to endocrine disruptor organochlorines and emerging contaminants in mediterranean cetaceans. MARINE ENVIRONMENTAL RESEARCH, 62, S200-S204.|
|Appare nelle tipologie:||1.1 Articolo in rivista|