It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chainrelated polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles

Cai, G., Moscatelli, A., Ciampolini, F., & Cresti, M. (1998). Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum. SEXUAL PLANT REPRODUCTION, 11(1), 31-40 [10.1007/s004970050118].

Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum

CAI, GIAMPIERO;CIAMPOLINI, FABRIZIO;CRESTI, MAURO
1998

Abstract

It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chainrelated polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles
Cai, G., Moscatelli, A., Ciampolini, F., & Cresti, M. (1998). Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum. SEXUAL PLANT REPRODUCTION, 11(1), 31-40 [10.1007/s004970050118].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/39666