A voltage-dependent K+ current has been revealed in whole-cell recordings carried out on immortalized cells obtained from the human tracheal epithelium. At positive membrane potentials the current shows a time dependent inactivation which is accelerated by increasing the depolarizing step. Forskolin, a direct activator of adenylyl cyclase, and verapamil, a Ca2+ channel blocker, induce the K+ current to inactivate more rapidly. Control experiments show that the action of these two compounds is not mediated by cyclic AMP and Ca2+. The application of 1,9-dideoxyforskolin, an analogue which does not stimulate adenylate cyclase, inhibits the current in the same way as forskolin; on the contrary, the dibutyryl analogue of cyclic AMP is ineffective. Furthermore, eliminating extracellular Ca2+ does not affect K+ current kinetics. Tetraethylammonium is an effective blocker of this current with an IC50 of 0.3 mM.
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|Titolo:||A forskolin and verapamil sensitive potassium current in human tracheal cells|
|Citazione:||L. J. V., G., A., R., Barone, V., D. C., G., & G., R. (1991). A forskolin and verapamil sensitive potassium current in human tracheal cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 179, 1155-1160.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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