32D cells are murine myeloid cells that grow indefinitely in Interleukin-3 (IL-3). In these cells, the type 1 insulin-like growth factor (IGF-I) and granulocytic-colony stimulating factor (G-CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate-1 (IRS-1) or IRS-2, docking proteins of the IGF-I receptor. Ectopic expression of IRS-1 in these cells inhibits differentiation, the cells become IL-3 independent and IGF-1 dependent and can form tumors in mice. 32D and 32D-derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro-arrays and identify the miR that are regulated by IGF-1 or G-CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells.

Shi, B., Prisco, M., Calin, G., Liu, C.G., Russo, G., Giordano, A., et al. (2006). Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells. JOURNAL OF CELLULAR PHYSIOLOGY, 207(3), 706-710 [10.1002/jcp.20613].

Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells

GIORDANO A.;
2006-01-01

Abstract

32D cells are murine myeloid cells that grow indefinitely in Interleukin-3 (IL-3). In these cells, the type 1 insulin-like growth factor (IGF-I) and granulocytic-colony stimulating factor (G-CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate-1 (IRS-1) or IRS-2, docking proteins of the IGF-I receptor. Ectopic expression of IRS-1 in these cells inhibits differentiation, the cells become IL-3 independent and IGF-1 dependent and can form tumors in mice. 32D and 32D-derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro-arrays and identify the miR that are regulated by IGF-1 or G-CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells.
2006
Shi, B., Prisco, M., Calin, G., Liu, C.G., Russo, G., Giordano, A., et al. (2006). Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells. JOURNAL OF CELLULAR PHYSIOLOGY, 207(3), 706-710 [10.1002/jcp.20613].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/38018
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