Macrophage migration inhibitory factor (MIF) is an ubiquitous protein playing various immunological and hormonal roles. Theoretical electrophoretic coordinates calculated from protein sequence in the SWISS-PROT database (AC P14174) are 12 kDa and pI 8.24. Using two-dimensional (2-D) immunoblotting, we have detected isoelectric forms at ca. 11.9 kDa, with pI values of 7.8 and 6.98 in human liver tissue, breast tissue and a cell line and in preparations of human MIF expressed in E. coli. This evidence suggests that MIF charge heterogeneity originates from a post-translational modification not requiring eukaryote-specific enzymes. We have also detected in human liver a minor immunoreactive spot at pI 6.23, which coincides with the MIF spot in the liver map in SWISS-2DPAGE. The pI 6.23 isoform also conceivably derives from post-translational modification, as MIF is known to be encoded in the human genome by a single copy gene.
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|Titolo:||Charge heterogeneity of macrophage migration inhibitory factor (MIF) in human liver and breast tissue.|
|Citazione:||Magi, B., Bini, L., Liberatori, S., Marzocchi, B., Raggiaschi, R., Arcuri, F., et al. (1998). Charge heterogeneity of macrophage migration inhibitory factor (MIF) in human liver and breast tissue. ELECTROPHORESIS, 19(11), 2010-2013.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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