Macrophage migration inhibitory factor (MIF) is an ubiquitous protein playing various immunological and hormonal roles. Theoretical electrophoretic coordinates calculated from protein sequence in the SWISS-PROT database (AC P14174) are 12 kDa and pI 8.24. Using two-dimensional (2-D) immunoblotting, we have detected isoelectric forms at ca. 11.9 kDa, with pI values of 7.8 and 6.98 in human liver tissue, breast tissue and a cell line and in preparations of human MIF expressed in E. coli. This evidence suggests that MIF charge heterogeneity originates from a post-translational modification not requiring eukaryote-specific enzymes. We have also detected in human liver a minor immunoreactive spot at pI 6.23, which coincides with the MIF spot in the liver map in SWISS-2DPAGE. The pI 6.23 isoform also conceivably derives from post-translational modification, as MIF is known to be encoded in the human genome by a single copy gene.

Magi, B., Bini, L., Liberatori, S., Marzocchi, B., Raggiaschi, R., Arcuri, F., et al. (1998). Charge heterogeneity of macrophage migration inhibitory factor (MIF) in human liver and breast tissue. ELECTROPHORESIS, 19(11), 2010-2013.

Charge heterogeneity of macrophage migration inhibitory factor (MIF) in human liver and breast tissue.

MAGI, BARBARA;BINI, LUCA;MARZOCCHI, BARBARA;ARCURI, FELICE;CINTORINO, MARCELLA;TOSI, PIERO;PALLINI, VITALIANO
1998-01-01

Abstract

Macrophage migration inhibitory factor (MIF) is an ubiquitous protein playing various immunological and hormonal roles. Theoretical electrophoretic coordinates calculated from protein sequence in the SWISS-PROT database (AC P14174) are 12 kDa and pI 8.24. Using two-dimensional (2-D) immunoblotting, we have detected isoelectric forms at ca. 11.9 kDa, with pI values of 7.8 and 6.98 in human liver tissue, breast tissue and a cell line and in preparations of human MIF expressed in E. coli. This evidence suggests that MIF charge heterogeneity originates from a post-translational modification not requiring eukaryote-specific enzymes. We have also detected in human liver a minor immunoreactive spot at pI 6.23, which coincides with the MIF spot in the liver map in SWISS-2DPAGE. The pI 6.23 isoform also conceivably derives from post-translational modification, as MIF is known to be encoded in the human genome by a single copy gene.
1998
Magi, B., Bini, L., Liberatori, S., Marzocchi, B., Raggiaschi, R., Arcuri, F., et al. (1998). Charge heterogeneity of macrophage migration inhibitory factor (MIF) in human liver and breast tissue. ELECTROPHORESIS, 19(11), 2010-2013.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/3757
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