Mammalian cell cycle progression is regulated by several protein kinases that are activated by cyclically expressed proteins called cyclins. These cyclin-dependent kinases, the prototype of which is the cdc2 mitosis-promoting kinase, are known to phosphorylate substrates the modified status of which is critical for the cell to progress into sequential phases of the cycle. Recently, a new cdc2-related protein kinase has been discovered, PISSLRE, named with respect to its homology to the cdc2 PSTAIRE amino acid domain. Here we report that by using both antisense and dominant-negative mutant constructs of PISSLRE when overexpressed in U20S cells, a growth suppression is found. Furthermore, the dominant negative forms of PISSLRE halt cell cycle progression in G2-M. Therefore, PISSLRE is essential for cellular proliferation, and its effect is exerted in G2-M. This describes the first evidence since cdc2 of a cdc2-related kinase acting through G2-M. © 1995, American Association for Cancer Research. All rights reserved.

Li, S., Maclachlan, T.K., De Luca, A., Claudio, P.P., Condorelli, G., Giordano, A. (1995). The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle. CANCER RESEARCH, 55(18), 3992-3995.

The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle

Giordano A.
1995-01-01

Abstract

Mammalian cell cycle progression is regulated by several protein kinases that are activated by cyclically expressed proteins called cyclins. These cyclin-dependent kinases, the prototype of which is the cdc2 mitosis-promoting kinase, are known to phosphorylate substrates the modified status of which is critical for the cell to progress into sequential phases of the cycle. Recently, a new cdc2-related protein kinase has been discovered, PISSLRE, named with respect to its homology to the cdc2 PSTAIRE amino acid domain. Here we report that by using both antisense and dominant-negative mutant constructs of PISSLRE when overexpressed in U20S cells, a growth suppression is found. Furthermore, the dominant negative forms of PISSLRE halt cell cycle progression in G2-M. Therefore, PISSLRE is essential for cellular proliferation, and its effect is exerted in G2-M. This describes the first evidence since cdc2 of a cdc2-related kinase acting through G2-M. © 1995, American Association for Cancer Research. All rights reserved.
1995
Li, S., Maclachlan, T.K., De Luca, A., Claudio, P.P., Condorelli, G., Giordano, A. (1995). The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle. CANCER RESEARCH, 55(18), 3992-3995.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/37054
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