Sinefungin (SFG) is ail antifungal and antiparasitic nucleoside antibiotic composed by ornithine and adenosine moieties both having the potential to bind copper(II). NMR studies performed at physiological pH have shown that the a-amino and the carboxylate groups in the ornithine unit are the preferred donor sites for Cu(II) binding. On the contrary, at acidic pH, Cu(II) complexation starts from adenosine nitrogen being the a-amino group still protonated and not available for metal binding. The proton paramagnetic relaxation enhancements measured at neutral pH allowed to obtain the 3D structure of the 1:2 Cu(II)-SFG complex. Molecular dynamics calculations were revealing for the existence of secondary Cu(II) interaction with the purine nitrogens of the adenosine moiety.

Cappannelli, M., Gaggelli, E., Jezowska Bojczuk, M., Molteni, E., Mucha, A., Porciatti, E., et al. (2007). 1H- and 13C-NMR study of the complex formed by Copper (II) with the Nucleoside Antibiotic Sinefungin. JOURNAL OF INORGANIC BIOCHEMISTRY, 101(7), 1005-1012 [10.1016/j.jinorgbio.2007.03.012].

1H- and 13C-NMR study of the complex formed by Copper (II) with the Nucleoside Antibiotic Sinefungin

Cappannelli, M.;Gaggelli, Elena;Valensin, Daniela;Valensin, Gianni
2007-01-01

Abstract

Sinefungin (SFG) is ail antifungal and antiparasitic nucleoside antibiotic composed by ornithine and adenosine moieties both having the potential to bind copper(II). NMR studies performed at physiological pH have shown that the a-amino and the carboxylate groups in the ornithine unit are the preferred donor sites for Cu(II) binding. On the contrary, at acidic pH, Cu(II) complexation starts from adenosine nitrogen being the a-amino group still protonated and not available for metal binding. The proton paramagnetic relaxation enhancements measured at neutral pH allowed to obtain the 3D structure of the 1:2 Cu(II)-SFG complex. Molecular dynamics calculations were revealing for the existence of secondary Cu(II) interaction with the purine nitrogens of the adenosine moiety.
2007
Cappannelli, M., Gaggelli, E., Jezowska Bojczuk, M., Molteni, E., Mucha, A., Porciatti, E., et al. (2007). 1H- and 13C-NMR study of the complex formed by Copper (II) with the Nucleoside Antibiotic Sinefungin. JOURNAL OF INORGANIC BIOCHEMISTRY, 101(7), 1005-1012 [10.1016/j.jinorgbio.2007.03.012].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/3637
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